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Acetylcholinesterase from Puntius javanicus for the detection of carbamates and organophosphates

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dc.Chemicals/CASacephate, 30560-19-1; acetylcholinesterase, 9000-81-1; carbamic acid, 463-77-4; carbaril, 63-25-2; carbofuran, 1563-66-2; chlorpyrifos, 2921-88-2; dimethoate, 60-51-5; dimpylate, 333-41-5; malathion, 121-75-5; methomyl, 16752-77-5; metrifonate, 52-68-6; parathion, 3270-86-8, 56-38-2, 597-88-6; procainamide, 51-06-9, 614-39-1; propoxur, 114-26-1
dc.citedby6
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Malaysia Sabah (UMS)
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsAsia Metropolitan University
dc.contributor.affiliationsUniversiti Malaysia Perlis (UniMAP)
dc.contributor.authorSabullah M.K.en_US
dc.contributor.authorAhmad S.A.en_US
dc.contributor.authorShukor M.Y.en_US
dc.contributor.authorShamaan N.A.en_US
dc.contributor.authorKhalid A.en_US
dc.contributor.authorGansau A.J.en_US
dc.contributor.authorDahalan F.A.en_US
dc.contributor.authorSulaiman M.R.en_US
dc.date.accessioned2024-05-28T08:33:10Z
dc.date.available2024-05-28T08:33:10Z
dc.date.issued2015
dc.description.abstractA new alternative local source of acetylcholinesterase (AChE) has been found to be sensitive towards several insecticides. AChE was purified from the brain tissue of Puntius javanicususing affinity chromatography (procainamide hydrochloride coupled with sephacryl 6B) with the total recovery of 40% at 8.48 purification fold. All carbamate (CB) insecticides tested at the concentration of 1 mg/L were capable of lowering AChE activity to less than 30%; Bendiocarp (18.80%), carbaryl (5.96%,), carbofuran (6.12%), methomyl (13.91%) and propoxur (15.851%). The P. javanicus AChE was found to be unaffected by several activated organophosphates (OP) such as acephate and dimethoate, while trichlorfon slightly inhibited the enzyme activity (p<0.05). Chlorpyrifos, diazinon, malathion and parathion lowered AChE activity to 43.02, 40.97, 37.11 and 46.72%, respectively. Pesticides that inhibit AChE activities more than 50% were further tested in different concentrations to determine the half maximal inhibitory concentration (IC50). Carbofuran and carbaryl showed the lowest IC50 value at 0.035 and 0.031 mg/L, respectively, as both showed no significant differences (p<0.05), followed by bendiocarp, propoxur, methomyl, malathion, diazinon, parathion and chlorpyrifos at 0.045, 0.076, 0.090, 0.063, 0.103, 0.151 and 0.202 mg/L, respectively. Based on these results, the sensitivity of AChE from brain P. javanicus brain tissue shows promise as an alternative biosensor for the detection of insecticides contamination.en_US
dc.description.natureFinalen_US
dc.identifier.epage353
dc.identifier.issn9742115
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84938572363
dc.identifier.spage348
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84938572363&partnerID=40&md5=e5bf039d9f2f1162bf8747d336e3aa97
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9049
dc.identifier.volume8
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherSPB Pharma Societyen_US
dc.relation.ispartofJournal of Chemical and Pharmaceutical Sciences
dc.sourceScopus
dc.subjectAcetylcholinesteraseen_US
dc.subjectCarbamateen_US
dc.subjectOrganophosphateen_US
dc.subjectPuntius javanicusen_US
dc.titleAcetylcholinesterase from Puntius javanicus for the detection of carbamates and organophosphatesen_US
dc.title.alternativeJ. Chem. Pharm. Sci.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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