Biodegradation of Methylene Blue Using Trichoderma reesei sp.

Rif'ah Sahira Sazali,; Liyana Amalina Adnan,; Ammar Ahmadi

Publication:
Biodegradation of Methylene Blue Using Trichoderma reesei sp.

dc.contributor.author	Rif'ah Sahira Sazali,	en_US
dc.contributor.author	Liyana Amalina Adnan,	en_US
dc.contributor.author	Ammar Ahmadi	en_US
dc.date.accessioned	2024-05-30T03:39:55Z
dc.date.available	2024-05-30T03:39:55Z
dc.date.issued	2021-02-18
dc.description	Junior Researcher International Conference (iJURECON) Kolej Genius Insan (KGI) 17 dan 18 November 2020 Pages : 234-237	en_US
dc.description.abstract	Trichoderma reesei sp is an anamorph fungi which involves asexual reproduction to form discrete anamorphs in mold like configuration. It also has high rate of mycelium growth of 100% and rate of methylene blue biodegradation of 100% by day 6 in culture medium. Since methylene blue dye is one of the dyes involved in dye pollution, fungi treatment is found as most immaculate compared to other technologies in our current timeline. Agar medium is first-handedly prepared in the laboratory which consists of nutrient agar (20g/L) and glucose (20g/L). At a subsequent time, second culture of agar medium is effectuated by adding methylene blue dye (50mg/L) in agar medium to calculate the rate of methylene blue dye decolourisation before proceeding with the physico-chemical parameters determination in terms of carbon source and nitrogen source for optimum growth in a conducive environment, ascertaining the rate of methylene blue decolourisation. Liquid medium is prepared prior to the investigation of the physico-chemical parameters. By keeping pH 8 and temperature of 37°c constant, the optimum carbon source is determined by contrasting the carbon source concentration (5g/100mL, 10g/100mL, 15g/100mL, 20g/100mL, 25g/100mL, 30g/100mL) corresponding to nitrogen source determination by also contrasting the nitrogen source concentration. Methylene blue decolourisation in optimum conditions is then analysed using UV-vis spectrophotometry.	en_US
dc.identifier.uri	https://oarep.usim.edu.my/handle/123456789/16394
dc.language.iso	en	en_US
dc.publisher	Universiti Sains Islam Malaysia	en_US
dc.relation.conference	iJURECON (KOLEJ GENIUS INSAN)	en_US
dc.subject	Trichoderma reesei sp.,	en_US
dc.subject	methylene blue dye,	en_US
dc.subject	carbon source,	en_US
dc.subject	nitrogen source,	en_US
dc.subject	UV-vis spectrophotometry	en_US
dc.title	Biodegradation of Methylene Blue Using Trichoderma reesei sp.	en_US
dspace.entity.type	Publication