Publication: High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley and Sons Ltd
Abstract
The bifunctional alcohol/aldehyde dehydrogenase (AdhE) comprises both an N-terminal aldehyde dehydrogenase (AldDH) and a C-terminal alcohol dehydrogenase (ADH). In vivo, full-length AdhE oligomerizes into long oligomers known as spirosomes. However, structural analysis of AdhE is challenging owing to the heterogeneity of the spirosomes. Therefore, the domains of AdhE are best characterized separately. Here, the structure of ADH from the pathogenic Escherichia coli O157:H7 was determined to 1.65 Å resolution. The dimeric crystal structure was confirmed in solution by small-angle X-ray scattering.
Keywords: alcohol dehydrogenase; AdhE; Escherichia coli
Description
Volume 76| Part 9| September 2020| Pages 414-421
Keywords
alcohol dehydrogenase;, AdhE;, Escherichia coli, alcohol dehydrogenase domain of AdhE,, 6sci;, NAD-bound,, 6scg