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In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia

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dc.contributor.authorKen, WKen_US
dc.contributor.authorRahman, MMen_US
dc.contributor.authorHanafiah, Aen_US
dc.contributor.authorIsahak, Ien_US
dc.date.accessioned2024-05-29T02:53:52Z
dc.date.available2024-05-29T02:53:52Z
dc.date.issued2012
dc.description.abstractObjectives: Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children worldwide. Its detection by viral culture and immunofluorescent assay is time consuming and has low sensitivity. The objective of the study was to develop In House Light Cycler Real Time Polymerase Chain Reaction for Rapid Detection of Respiratory Syncytial Virus. Methodology: In house developed Light Cycler Real-Time reverse transcriptase polymerase chain reaction (LC-RT-PCR) assay was standardized using designed primers for the rapid identification of clinical samples of RSV. Results: A total of 130 samples (throat swab and nasopharyngeal aspirate) obtained from University Kebangsaan Malaysia Medical Centre, were efficiently tested and of which 54 (42%) were RSV positive. Conclusion: This study indicated that this newly developed in house rRT-PCR assay proved to be an excellent method for the rapid detection of RSV.
dc.identifier.epage822
dc.identifier.issn1682-024X
dc.identifier.issue5
dc.identifier.scopusWOS:000309409600010
dc.identifier.spage818
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11429
dc.identifier.volume28
dc.languageEnglish
dc.language.isoen_US
dc.publisherProfessional Medical Publicationsen_US
dc.relation.ispartofPakistan Journal Of Medical Sciences
dc.sourceWeb Of Science (ISI)
dc.subjectRespiratory Syncytial virusen_US
dc.subjectLightCycler real time polymerase chain reactionen_US
dc.subjectPrimeren_US
dc.subjectSequenceen_US
dc.subjectProbe and Plasmiden_US
dc.titleIn-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia
dc.typeArticleen_US
dspace.entity.typePublication

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