Identification of Antioxidants and Active Compounds in Cow’s Milk

This paper aims to analyze and identify the antioxidant compounds in cow’s milk. Milk contains high biological molecules protein, essential fatty acids, calcium, fat, amino acid, vitamins that are soluble in water and several bioactive compounds. In Al-Quran and Hadith, milk has been mentioned several times especially for breast feeding which is essential for infant immunity. Antioxidant can suppress free radicals and unstable molecules produced by the body as an environmental and other pressure reaction. In this research, the commercialized milks were successfully extracted by liquid-liquid extraction method. Therefore, this study focuses on the identification of antioxidant compounds in commercialized milk samples using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR) and Gas ChromatographyMass Spectroscopy (GC-MS). Based on the ATR-FTIR spectrum, we are able to identify the functional groups of antioxidant compounds which are C–H stretch of alkanes, C=O of aldehydes, C–N stretch of aliphatic amine, C–Cl stretch of alkyl halide, O–H bend of carboxylic acids and N–H wag of 1 , 2 amines. Further, the spectrum of the chromatogram from the GCMS can determine the antioxidants in the milk samples. This study enables the dairy product factory to improve their quality of the milk to enhance the consumers’ health.