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An alternative bioassay using Anabas testudineus (Climbing perch) colinesterase for metal ions detection

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dc.contributor.authorAhmad, SAen_US
dc.contributor.authorWong, YFen_US
dc.contributor.authorShukor, MYen_US
dc.contributor.authorSabullah, MKen_US
dc.contributor.authorYasid, NAen_US
dc.contributor.authorHayat, NMen_US
dc.contributor.authorShamaan, NAen_US
dc.contributor.authorKhalid, Aen_US
dc.contributor.authorSyed, MAen_US
dc.date.accessioned2024-05-29T02:49:43Z
dc.date.available2024-05-29T02:49:43Z
dc.date.issued2016
dc.description.abstractClimbing Perch or its scientific name, Anabas testudineus is one of the freshwater fish belonging to the family of Anabantidae. It is widely distributed in ponds, swamps and estuaries in Asia. In this study, cholinesterase (ChE) was partially purified from the liver of A. testudineus through ion exchange chromatography. This purification method provided a recovery yield of 5.36% with a purification fold of 6.6. The optimum conditions for ChE assay were identified to be 2.5 mM of butyrylthiocholine iodide (BTC) with pH 8.0 in Tris-HCl buffer at 40 degrees C. Substrate specificity profile also indicated that ChE favours BTC as substrate because it records the highest catalytic efficiency (V-max/K-m). Protein analysis through Native-PAGE showed that ion exchange chromatography is an effective method to partially purify ChE. Metal ion inhibition tests were conducted and mercury (Hg) was found to show the highest inhibition effect (87.30%) whereas lead (Pb) shows the lowest inhibition effect (28.01%). All these findings showed that partially purified ChE from the liver of A. testudineus is suitable to be used as a bioindicator to detect the presence of metal ions. (c) All Rights Reserved
dc.identifier.epage1452
dc.identifier.isbn2231-7546
dc.identifier.issn1985-4668
dc.identifier.issue4
dc.identifier.scopusWOS:000423032300014
dc.identifier.spage1446
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10916
dc.identifier.volume23
dc.languageEnglish
dc.language.isoen_US
dc.publisherUniv Putra Malaysia Pressen_US
dc.relation.ispartofInternational Food Research Journal
dc.sourceWeb Of Science (ISI)
dc.subjectBioindicatoren_US
dc.subjectCholinesterase (ChE)en_US
dc.subjectInhibitionen_US
dc.subjectMetal ionsen_US
dc.titleAn alternative bioassay using Anabas testudineus (Climbing perch) colinesterase for metal ions detection
dc.typeArticleen_US
dspace.entity.typePublication

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