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Occurance of Blastocystis sp in water catchments at Malay villages and Aboriginal settlement during wet and dry seasons in Peninsular Malaysia

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dc.contributor.authorNoradilah, SAen_US
dc.contributor.authorLee, ILen_US
dc.contributor.authorAnuar, TSen_US
dc.contributor.authorSalleh, FMen_US
dc.contributor.authorManap, SNAAen_US
dc.contributor.authorMohtar, NSHMen_US
dc.contributor.authorAzrul, SMen_US
dc.contributor.authorAbdullah, WOen_US
dc.contributor.authorMoktar, Nen_US
dc.date.accessioned2024-05-29T02:57:15Z
dc.date.available2024-05-29T02:57:15Z
dc.date.issued2016
dc.description.abstractIn the tropics, there are too few studies on isolation of Biastocystis sp. subtypes from water sources; in addition, there is also an absence of reported studies on the occurrence of Blastocystis sp. subtYPes In water during different seasons. Therefore, this stud)/ was aimed to determine the occurrence of Blastocystis sp. subtypes in river water and other water sources that drained aboriginal vicinity of highly endemic intestinal parasitic infections during wet and dry seasons. Water samples were collected from six sampling Points of Sungai Krau (K1-K6) and a Point at Sungai Lompat (K7) and ()the: water sources around the aboriginal villages. The water samples were collected during both seasons, wet and dry seasons. Filtration of the water samples were carried out using a flatbed membrane filtration system. The extracted DNA from concentrated water sediment was subjected to single round polymerase chain reaction and positive PCR products were subjected to sequencing. All samples were also subjected to filtration and cultured on membrane lactose glucuronide agar for the detection of faecal coliforms. During wet season, Blastocystis sp. ST1, ST2 and ST3 were detected in river water samples. Blastocystis sp. ST3 occurrence was sustained in the river water samples during dry season. However Blastocystis sp. ST1 and ST2 were absent during dry season. Water samples collected from various water sources showed contaminations of Blastocystis sp. ST1, ST2, ST3 and ST4, during wet season and Blastocystis sp. ST1, ST3, ST8 and ST10 during dry season. Water collected from all river sampling points during both seasons showed growth of Escherichia coli and Enterobacter aerogenes, indicating faecal contamination. In this study, Blastocystis sp. ST3 is suggested as the most resistant subtype able to survive in any adverse environmental condition. robust and Restriction and control of human and animal faecal contaminations to the river and other water
dc.identifier.doi10.7717/peerj.2541
dc.identifier.issn2167-8359
dc.identifier.scopusWOS:000385578600007
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11628
dc.identifier.volume4
dc.languageEnglish
dc.language.isoen_US
dc.publisherPeerj Incen_US
dc.relation.ispartofPeerj
dc.sourceWeb Of Science (ISI)
dc.subjectBlastocystis sp.en_US
dc.subjectWateren_US
dc.subjectAboriginal settlementsen_US
dc.subjectWet and dry seasonsen_US
dc.titleOccurance of Blastocystis sp in water catchments at Malay villages and Aboriginal settlement during wet and dry seasons in Peninsular Malaysia
dc.typeArticleen_US
dspace.entity.typePublication

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