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The identification and detection of a unique gene in Salmonella enterica subsp. enterica Weltevreden using PCR-based approach

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dc.contributor.affiliationsIslamic Science Institute
dc.contributor.affiliationsUniversiti Sains Malaysia (USM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorTan C.en_US
dc.contributor.authorYuan Xin G.en_US
dc.contributor.authorChiann Ying Y.en_US
dc.contributor.authorIsmail A.en_US
dc.contributor.authorKia Kien P.en_US
dc.date.accessioned2024-05-28T08:41:31Z
dc.date.available2024-05-28T08:41:31Z
dc.date.issued2014
dc.description.abstractIntroduction: Salmonella enterica are important food-borne pathogens that cause human gastroenteritis, bacteremia and subsequent focal infections. The incidences of food-borne salmonellosis due to non-typhoidal Salmonella serotypes are increasing periodically in Malaysia. Other than the two predominant non-typhoidal Salmonella serotypes, S. Typhimurium and S. Enteritidis, an emerging serotype, S. Weltevreden, is increasingly being isolated from hospitalized-gastroenteritis patients in Malaysia. Serotyping is the conventional method for differentiation of this pathogen from the 2500 other serotypes known to exist. However, this method has a number of disadvantages including low specificity and high cost. Therefore, a molecular method for identification of S. Weltevreden in clinical isolates based on amplification of a specific DNA sequence present in its genome is proposed. Methods: A total of 234 clinical isolates were collected from the Department of Clinical Microbiology and Parasitology, HUSM from year 2010-2012. There were 49 S. Weltevreden isolates which have been confirmed by the Institute of Medical Research (IMR) using conventional serotyping method. Two sets of primers targeting a unique gene in S. Weltevreden (SWE349) and a pan- Salmonella gene (invA284) were designed using Primer3 online software, and a multiplex-PCR (mPCR) assay was developed to detect S. Weltevreden in clinical isolates. The optimized S. Weltevreden mPCR assay was evaluated on a panel of known Salmonella and non- Salmonella spp. to determine the specificity and sensitivity of the mPCR assay. Results & Discussion: The S. Weltevreden serovar specific primer, SWE349, and the internal control primer, invA284, resulted in 2 PCR products of 349bp and 284bp, respectively. The mPCR assay for S. Weltevreden targeting gene SWE349 showed 100% sensitivity (49/49) and specificity (36/36) in concordance with the conventional serotyping method in detecting only S. Weltevreden clinical strains, but not from the panel of 26 non-typhoidal Salmonella and 10 non- Salmonella isolates. Conclusion: A cost effective, sensitive and specific mPCR assay has been developed for the detection and confirmation of Salmonella Weltevreden from clinical isolates of gastroenteritis patients, using genes SWE349 and invA284. � 2014 Asian Pacific Tropical Medicine Press.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.1016/S2222-1808(14)60516-8
dc.identifier.issn22221808
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84896696628
dc.identifier.spage226
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84896696628&doi=10.1016%2fS2222-1808%2814%2960516-8&partnerID=40&md5=a27cfbfa98119d0959395a3b9c5751de
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9298
dc.identifier.volume4
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofOpen Accessen_US
dc.relation.ispartofAsian Pacific Journal of Tropical Disease
dc.sourceScopus
dc.titleThe identification and detection of a unique gene in Salmonella enterica subsp. enterica Weltevreden using PCR-based approachen_US
dc.title.alternativeAsian Pac. J. Trop. Dis.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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