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Helicobacter pylori eradication causes perturbation of the human gut microbiome in young adults

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dc.Chemicals/CASamoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; clarithromycin, 81103-11-9; pantoprazole, 102625-70-7; Anti-Bacterial Agents
dc.citedby45
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversity of Malaya (UM)
dc.contributor.affiliationsMonash University Malaysia
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsNew York University Cancer Institute
dc.contributor.affiliationsNew York University
dc.contributor.authorYap T.W.-C.en_US
dc.contributor.authorGan H.-M.en_US
dc.contributor.authorLee Y.-P.en_US
dc.contributor.authorLeow A.H.-R.en_US
dc.contributor.authorAzmi A.N.en_US
dc.contributor.authorFrancois F.en_US
dc.contributor.authorPerez-Perez G.I.en_US
dc.contributor.authorLoke M.-F.en_US
dc.contributor.authorGoh K.-L.en_US
dc.contributor.authorVadivelu J.en_US
dc.date.accessioned2024-05-28T08:38:20Z
dc.date.available2024-05-28T08:38:20Z
dc.date.issued2016
dc.description.abstractBackground: Accumulating evidence shows that Helicobacter pylori protects against some metabolic and immunological diseases in which the development of these diseases coincide with temporal or permanent dysbiosis. The aim of this study was to assess the effect of H. pylori eradication on the human gut microbiome. Methods: As part of the currently on-going ESSAY (Eradication Study in Stable Adults/Youths) study, we collected stool samples from 17 H. pylori-positive young adult (18-30 years-old) volunteers. The same cohort was followed up 6,12 and 18 months-post H. pylori eradication. The impact of H. pylori on the human gut microbiome pre- and post-eradication was investigated using high throughput 16S rRNA gene (V3-V4 region) sequencing using the Illumina Miseq followed by data analysis using Qiime pipeline. Results: We compared the composition and diversity of bacterial communities in the fecal microbiome of the H. pylori-positive volunteers, before and after H. pylori eradication therapy. The 16S rRNA gene was sequenced at an average of 150,000-170,000 reads/sample. The microbial diversity were similar pre- and post-H. pylori eradication with no significant differences in richness and evenness of bacterial species. Despite that the general profile of the gut microbiome was similar pre- and post-eradication, some changes in the bacterial communities at the phylum and genus levels were notable, particularly the decrease in relative abundance of Bacterioidetes and corresponding increase in Firmicutes after H. pylori eradication. The significant increase of short-chain fatty acids (SCFA)-producing bacteria genera could also be associated with increased risk of metabolic disorders. Conclusions: Our preliminary stool metagenomics study shows that eradication of H. pylori caused perturbation of the gut microbiome and may indirectly affect the health of human. Clinicians should be aware of the effect of broad spectrum antibiotics used in H. pylori eradication regimen and be cautious in the clinical management of H. pylori infection, particularly in immunocompromised patients. � 2016 Yap et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.description.natureFinalen_US
dc.identifier.ArtNoe0151893
dc.identifier.CODENPOLNC
dc.identifier.doi10.1371/journal.pone.0151893
dc.identifier.issn19326203
dc.identifier.issue3
dc.identifier.pmid26991500
dc.identifier.scopus2-s2.0-84962159012
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84962159012&doi=10.1371%2fjournal.pone.0151893&partnerID=40&md5=2ab4f6071ed32a00474a009b6593044c
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9211
dc.identifier.volume11
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofOpen Accessen_US
dc.relation.ispartofPLoS ONE
dc.sourceScopus
dc.titleHelicobacter pylori eradication causes perturbation of the human gut microbiome in young adultsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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