Publication:
A Point-of-Care Immunosensor for Human Chorionic Gonadotropin in Clinical Urine Samples Using a Cuneated Polysilicon Nanogap Lab-on-Chip

Simple item page
dc.contributor.authorSharma Rao A/l Balakrishnanen_US
dc.contributor.authorU. Hashimen_US
dc.contributor.authorSubash C. B. Gopinathen_US
dc.contributor.authorP. Poopalanen_US
dc.contributor.authorH. R. Ramayyaen_US
dc.contributor.authorM. Iqbal Omaren_US
dc.contributor.authorR. Haarindraprasaden_US
dc.contributor.authorP. Veeradasanen_US
dc.date.accessioned2024-05-28T04:22:00Z
dc.date.available2024-05-28T04:22:00Z
dc.date.issued2015
dc.description.abstractHuman chorionic gonadotropin (hCG), a glycoprotein hormone secreted from the placenta, is a key molecule that indicates pregnancy. Here, we have designed a cost-effective, label-free, in situ point-of-care (POC) immunosensor to estimate hCG using a cuneated 25 nm polysilicon nanogap electrode. A tiny chip with the dimensions of 20.5 × 12.5 mm was fabricated using conventional lithography and size expansion techniques. Furthermore, the sensing surface was functionalized by (3-aminopropyl)triethoxysilane and quantitatively measured the variations in hCG levels from clinically obtained human urine samples. The dielectric properties of the present sensor are shown with a capacitance above 40 nF for samples from pregnant women; it was lower with samples from non-pregnant women. Furthermore, it has been proven that our sensor has a wide linear range of detection, as a sensitivity of 835.88 μA mIU-1 ml-2 cm-2 was attained, and the detection limit was 0.28 mIU/ml (27.78 pg/ml). The dissociation constant Kd of the specific antigen binding to the anti-hCG was calculated as 2.23 ± 0.66 mIU, and the maximum number of binding sites per antigen was Bmax = 22.54 ± 1.46 mIU. The sensing system shown here, with a narrow nanogap, is suitable for high-throughput POC diagnosis, and a single injection can obtain triplicate data or parallel analyses of different targets.en_US
dc.identifier.citationBalakrishnan SR, Hashim U, Gopinath SCB, Poopalan P, Ramayya HR, Iqbal Omar M, et al. (2015) A Point-of-Care Immunosensor for Human Chorionic Gonadotropin in Clinical Urine Samples Using a Cuneated Polysilicon Nanogap Lab-on-Chip. PLoS ONE 10(9): e0137891. doi:10.1371/journal. pone.0137891en_US
dc.identifier.doi10.1371/journal.pone.0137891
dc.identifier.epage17
dc.identifier.issn1932-6203
dc.identifier.issue9
dc.identifier.other2112-3
dc.identifier.spage1
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0137891
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/5384
dc.identifier.volume10
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPlos Oneen_US
dc.titleA Point-of-Care Immunosensor for Human Chorionic Gonadotropin in Clinical Urine Samples Using a Cuneated Polysilicon Nanogap Lab-on-Chipen_US
dc.typeArticleen_US
dspace.entity.typePublication

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
A Point-of-Care Immunosensor for Human Chorionic Gonadotropin in Clinical Urine Samples Using a Cuneated Polysilicon Nanogap Lab-on-Chip.pdf
Size:
1.79 MB
Format:
Adobe Portable Document Format
Download

Collections

Other Publications