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Gold-nanorod enhances dielectric voltammetry detection of c-reactive protein: A predictive strategy for cardiac failure

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dc.contributor.authorLetchumanan, Ien_US
dc.contributor.authorArshad, MKMen_US
dc.contributor.authorBalakrishnan, SRen_US
dc.contributor.authorGopinath, SCBen_US
dc.date.accessioned2024-05-29T03:00:55Z
dc.date.available2024-05-29T03:00:55Z
dc.date.issued2019
dc.descriptionBiosensors and Bioelectronics Volume 130, 1 April 2019, Pages 40-47en_US
dc.description.abstractThis paper primarily demonstrates the approach to enhance the sensing performance on antigen C-reactive protein (CRP) and anti-CRP antibody binding event. A nanogapped electrode structure with the gap of similar to 100 nm was modified by the anti-CRP antibody (Probe) to capture the available CRP. In order to increase the amount of antigen to be captured, a gold nanorod with 119 nm in length and 25 nm in width was integrated, to increase the surface area. A comparative study between the existence and non-existence of gold nanorod utilization was evaluated. Analysis of the sensing surface was well-supported by atomic force microscopy, scanning electron microscopy, 3D nano-profilometry, high-power microscopy and UV-Vis spectroscopy. The dielectric voltammetric analysis was carried out from 0 V to 2 V. The sensitivity was calculated based on 3 sigma and attained as low as 1 pM, which is tremendously low compared to real CRP concentration (119 nM) in human blood serum. The gold nanorod conjugation with antibody has enhanced the sensitivity to 100 folds (10 fM). The specificity of the CRP detection by the proposed strategy was anchored by ELISA and failure in the detection of human blood clotting factor IX by voltammetry. Despite, CRP antigen was further detected in human serum by spiking CRP to runthrough the detection with the physiologically relevant samples.en_US
dc.identifier.doi10.1016/j.bios.2019.01.042
dc.identifier.epage47
dc.identifier.isbn1873-4235
dc.identifier.issn0956-5663
dc.identifier.scopusWOS:000461526200003
dc.identifier.spage40
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85060888607&doi=10.1016%2fj.bios.2019.01.042&partnerID=40&md5=f67d9c6108f078db405ce9d8d0669139
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0956566319300715?via%3Dihub
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11803
dc.identifier.volume130
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherElsevier Advanced Technologyen_US
dc.relation.ispartofBiosensors and Bioelectronicsen_US
dc.sourceWeb Of Science (ISI)
dc.subjectC-reactive proteinen_US
dc.subjectGold nanoroden_US
dc.subjectNanogapped sensoren_US
dc.subjectVoltammetryen_US
dc.titleGold-nanorod enhances dielectric voltammetry detection of c-reactive protein: A predictive strategy for cardiac failureen_US
dc.typeArticleen_US
dspace.entity.typePublication

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