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Cerapan mikroskopi dan analisis sitometri Streptococcus mutans yang ditindakkan dengan ekstrak batang Melastoma malabathricum L

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dc.citedby1
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorRohazila M.H.en_US
dc.contributor.authorNazlina I.en_US
dc.contributor.authorYaacob W.A.en_US
dc.date.accessioned2024-05-29T02:03:32Z
dc.date.available2024-05-29T02:03:32Z
dc.date.issued2014
dc.descriptionMalays. Appl. Biol. (2014) 43(2): 41–47en_US
dc.description.abstractStreptococcus mutans is the earliest bacteria that forms dental caries in humans. This study determines the effect of acetone extract from Melastoma malabathricum stem against S. mutans by microscopic observation and absorption of fluorescence dye. The extraction of M. malabathricum stem using acetone yielded 0.5 g of acetone extract. Anti-bacterial activity determined by disk diffusion assay showed that stem extract at the 30 mg/mL inhibit bacterial growth with inhibition zone diameter of 15 mm. The effect of the extract towards S. mutans by microscopic observation was done by Scanning Electron Microscope (SEM) and Transmission Electronic Microscope (TEM). Result from microscopic observation showed that the M. malabathricum extract damaged the cell�s shape by rupturing the surface and the wall, causing the cytoplasm to leak. The assay using propidium iodide (PI) as the fluorescence dye showed that it was absorbed into the observed cell through flow cytometry analysis. The absorption of propidium iodide in treated cell confirmed the possibility of disturbance in the permeability of the cell membrane�s structure. From the microscopic observation and cytometry analysis, it can be concluded that acetone extract of M. malabathricum act as anti-bacteria agent that cause damage to the cell wall and disturb the permeability of the membrane structure. � 2014, Malaysian Society of Applied Biology. All rights reserved.en_US
dc.description.natureFinalen_US
dc.identifier.epage48
dc.identifier.issn1268643
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84920946342
dc.identifier.spage41
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84920946342&partnerID=40&md5=2e762a472c751e226797ac5f194d2cdf
dc.identifier.urihttp://www.mabjournal.com/images/43_2_Dec_2014/43_2_06.pdf
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10261
dc.identifier.volume43
dc.languageMalay
dc.language.isoen_USen_US
dc.publisherMalaysian Society of Applied Biologyen_US
dc.relation.ispartofMalaysian Applied Biologyen_US
dc.sourceScopus
dc.subjectCytometry analysisen_US
dc.subjectMicroscopic observationen_US
dc.subjectStreptococcus mutans and melastoma malabathricumen_US
dc.subjectBacteria (microorganisms)en_US
dc.subjectMelastoma malabathricumen_US
dc.subjectStreptococcus mutansen_US
dc.titleCerapan mikroskopi dan analisis sitometri Streptococcus mutans yang ditindakkan dengan ekstrak batang Melastoma malabathricum Len_US
dc.typeArticleen_US
dspace.entity.typePublication

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Cerapan Mikroskopi Dan Analisis Sitometri Streptococcus Mutans Yang Ditindakkan Dengan Ekstrak Batang Melastoma Malabathricum L.
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