Publication:
Inhibition Of Streptococcus Mutans Adherence And Biofilm Formation Activities From Melastoma Malabathricum Subfraction

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dc.contributor.authorRohazila Mohamad Hanafiahen_US
dc.contributor.authorSiti Aisyah Abd Ghafaren_US
dc.contributor.authorWan Ahmad Yaacoben_US
dc.contributor.authorWan Syaidatul Aqmaen_US
dc.contributor.authorNazlina Ibrahimen_US
dc.date.accessioned2024-05-27T14:48:37Z
dc.date.available2024-05-27T14:48:37Z
dc.date.issued2018
dc.descriptionVolume: 6, Issue: 4en_US
dc.description.abstractObjectives: The objectives of the study were to determine antibacterial, anti-adherence, and antibiofilm ctivities of Melastoma malabathricum stem bark acetone extract (MMSBAE) subfraction against Streptococcus mutans. Methods: Fraction 9 (F9) from MMSBAE was subfractionated by thin-layer chromatography (TLC) and analyzed for antibacterial activity against S. mutans by TLC-bioautography. Subfraction 12 (SF12) was isolated from F9 followed by determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. Results: MIC and MBC values were 10 mg/mL and 160 mg/mL, indicating bacteriostatic property of SF12. Time-kill assay analysis confirmed bacteriostatic property of SF12 against S. mutans. Crystal violet staining and glass surface assays were used to determine anti-adherence and antibiofilm activities. Concentrations produced 50% reduction in anti-adherence and antibiofilm activities were 40 mg/mL and 20 mg/mL, respectively. Scanning electron microscopy was performed to visualize the effect of SF12 on S. mutans biofilm structure. SF12 was found to lyse biofilm formation on treated bacteria indicating powerful anticariogenic potential against S. mutans. Analysis by quantitative real-time polymerase chain reaction revealed SF12 at MIC value downregulated biofilm formation genes such as gbpA, brpA, gtfC, and comDE. Conclusion: SF12 showed bacteriostatic activities against S. mutans by inhibiting adherence and biofilm activities.en_US
dc.identifier.epage4
dc.identifier.issn2321-550X
dc.identifier.issue4
dc.identifier.spage1
dc.identifier.urihttps://innovareacademics.in/journals/index.php/ijls/issue/view/406
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/3803
dc.identifier.volume6
dc.language.isoen_USen_US
dc.publisherInnovare Academic Sciencesen_US
dc.relation.ispartofInnovare Journal of Life Scienceen_US
dc.subjectStreptococcus mutans, Subfraction, Melastoma malabathricum stem bark acetone extract, Antibiofilm activity, Anti-adherence activityen_US
dc.titleInhibition Of Streptococcus Mutans Adherence And Biofilm Formation Activities From Melastoma Malabathricum Subfractionen_US
dc.typeArticleen_US
dspace.entity.typePublication

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