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Species Identification of Potential Probiotic Lactic Acid Bacteria Isolated from Malaysian Fermented Food Based on 16s Ribosomal RNA (16S rRNA) and Internal Transcribed Spacer (ITS) Sequences

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dc.contributor.authorYaacob Ilyanie
dc.contributor.authorNur Huda Faujan
dc.contributor.authorMd Yasin Ida Muryany
dc.date.accessioned2024-06-16T06:32:42Z
dc.date.available2024-06-16T06:32:42Z
dc.date.issued2023
dc.date.submitted2024-1-23
dc.descriptionMalaysian Applied Biology Volume: 52 Issue: 4(page: 73-84)
dc.description.abstractTaxonomic identification of potential probiotic lactic acid bacteria (LAB) in fermented foods is essential as the microorganisms’ benefits are varied and often strain-specific. Using biochemical and physiological methods alone is inadequate to precisely distinguish each strain. In this study, molecular techniques were employed in the identification of 17 LAB isolated from three Malaysian fermented foods: belacan (BE), bosou (BO), and budu (BUM). The respective sizes of polymerase chain reaction (PCR) products from the isolates were approximately 1500 bp and 750 bp when amplified with the 16S ribosomal RNA (16S rRNA) and internal transcribed spacer (ITS) gene primers. The phylogenetic analysis using both gene sequences revealed that all BE and BO isolates were identified as Lactiplantibacillus plantarum, while all BUM isolates were identified as Lacticaseibacillus paracasei. Both 16S rRNA and ITS genes could disclose the identity of the isolates up to the species level. In summary, the use of the ITS gene in conjunction with the 16S rRNA gene can help with the more effective identification of potential probiotic LAB strains isolated from fermented food.
dc.identifier.citationYaacob Ilyanie, Nur Huda Faujan, & Muryany, I. (2023). Species Identification of Potential Probiotic Lactic Acid Bacteria Isolated from Malaysian Fermented Food Based on 16S Ribosomal RNA (16S rRNA) and Internal Transcribed Spacer (ITS) Sequences. Malaysian Applied Biology/Malaysian Applied Biology Journal, 52(4), 73–84. https://doi.org/10.55230/mabjournal.v52i4.c146
dc.identifier.doi10.55230/mabjournal.v52i4.c146
dc.identifier.epage84
dc.identifier.issn0126-8643
dc.identifier.issue4
dc.identifier.spage73
dc.identifier.urihttps://www.jms.mabjournal.com/index.php/mab/article/view/2850
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/19754
dc.identifier.volume52
dc.language.isoen_US
dc.publisherMalaysian Society of Applied Biology
dc.relation.ispartofMalaysian Applied Biology
dc.relation.issn2462-151X
dc.relation.journalMalaysian Applied Biology
dc.subject16S rRNA gene
dc.subjectITS gene
dc.subjectlactic acid bacteria
dc.subjectMalaysian fermented food
dc.subjectPCR
dc.titleSpecies Identification of Potential Probiotic Lactic Acid Bacteria Isolated from Malaysian Fermented Food Based on 16s Ribosomal RNA (16S rRNA) and Internal Transcribed Spacer (ITS) Sequences
dc.typetext::journal::journal article::research article
dspace.entity.typePublication
oaire.citation.endPage84
oaire.citation.issue4
oaire.citation.startPage73
oaire.citation.volume52
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#

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