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Cytotoxic Activity of Kenaf Seed Oils from Supercritical Carbon Dioxide Fluid Extraction towards Human Colorectal Cancer (HT29) Cell Lines

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dc.contributor.authorSiti Aisyah Abd Ghafaren_US
dc.contributor.authorMaznah Ismailen_US
dc.contributor.authorLatifah Saiful Yazanen_US
dc.contributor.authorSharida Fakurazien_US
dc.contributor.authorNorsharina Ismailen_US
dc.contributor.authorKim Wei Chanen_US
dc.contributor.authorParidah Md Tahiren_US
dc.date.accessioned2024-05-28T03:32:44Z
dc.date.available2024-05-28T03:32:44Z
dc.date.issued2013
dc.date.submitted--
dc.description.abstractKenaf (Hibiscus cannabinus) from the family Malvaceae, is a valuable fiber plant native to India and Africa and is currently plantedas the fourth commercial crop in Malaysia. Kenaf seed oil contains alpha-linolenic acid, phytosterol such as𝛽-sitosterol, vitamin E, and other antioxidants with chemopreventive properties. Kenaf seeds oil (KSO) was from supercritical carbon dioxide extraction fluid (SFE) at 9 different permutations of parameters based on range of pressures from 200 to 600 bars and temperature from 40to 80∘C. They were 200/40, 200/60, 200/80, 400/40, 400/60, 400/80, 600/40, 600/60, and 600/80. Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast(NIH/3T3) cell lines using MTS assay. KSO-SFE at 600/40 showed the strongest cytotoxicity towards HT29 with IC50of 200𝜇g/mL. The IC50for NIH/3T3 was not detected even at highest concentration employed. Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. Further apoptosis induction was confirmed by Annexin V/PI and AO/PI staining.en_US
dc.identifier.doihttp://dx.doi.org/10.1155/2013/549705
dc.identifier.epage8
dc.identifier.issn1741-427X
dc.identifier.spage1
dc.identifier.urihttps://www.hindawi.com/journals/ecam/2013/549705/
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/4641
dc.identifier.volume2013
dc.language.isoen_USen_US
dc.publisherHindawi Publishing Corporationen_US
dc.relation.ispartofEvidence-Based Complementary and Alternative Medicineen_US
dc.titleCytotoxic Activity of Kenaf Seed Oils from Supercritical Carbon Dioxide Fluid Extraction towards Human Colorectal Cancer (HT29) Cell Linesen_US
dc.typeArticleen_US
dspace.entity.typePublication

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