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Disruption of methicillin-resistant Staphylococcus aureus protein synthesis by tannins

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dc.Chemicals/CASampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; cefoxitin, 33564-30-6, 35607-66-0; chloramphenicol, 134-90-7, 2787-09-9, 56-75-7; cotrimoxazole, 8064-90-2; erythromycin, 114-07-8, 70536-18-4; kanamycin, 11025-66-4, 61230-38-4, 8063-07-8; methanol, 67-56-1; oxacillin, 1173-88-2, 66-79-5, 7240-38-2; rifampicin, 13292-46-1; tetracycline, 23843-90-5, 60-54-8, 64-75-5, 8021-86-1; vancomycin, 1404-90-6, 1404-93-9
dc.FundingDetailsMinistry of Higher Education, Malaysia,�MOHE: UKM-ST-FRGS0110-2009 NMRR-11-59-8309
dc.FundingDetailsFunding: This study was financed by Ministry of Health Malaysia under grant number NMRR-11-59-8309 except for the tannins preparation which was supported by Ministry of Higher Education Malaysia under grant number UKM-ST-FRGS0110-2009.
dc.citedby3
dc.contributor.affiliationsFaculty of Dentistry
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.authorAdnan S.-N.-A.en_US
dc.contributor.authorIbrahim N.en_US
dc.contributor.authorYaacob W.A.en_US
dc.date.accessioned2024-05-29T01:55:03Z
dc.date.available2024-05-29T01:55:03Z
dc.date.issued2017
dc.description.abstractIntroduction Methicillin-resistant Staphylococcus aureus (MRSA) is a worldwide public health threat, displaying multiple antibiotic resistance that causes morbidity and mortality. Management of multidrug-resistant (MDR) MRSA infections is extremely difficult due to their inherent resistance to currently used antibiotics. New antibiotics are needed to combat the emergence of antimicrobial resistance. Methods The in vitro effect of tannins was studied against MRSA reference strain (ATCC 43300) and MRSA clinical strains utilizing antimicrobial assays in conjunction with both scanning and transmission electron microscopy. To reveal the influence of tannins in MRSA protein synthesis disruption, we utilized next-generation sequencing (NGS) to provide further insight into the novel protein synthesis transcriptional response of MRSA exposed to these compounds. Results Tannins possessed both bacteriostatic and bactericidal activity with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 0.78 and 1.56 mg/mL, respectively, against all tested MRSA. Scanning and transmission electron microscopy of MRSA treated with tannins showed decrease in cellular volume, indicating disruption of protein synthesis. Conclusion Analysis of a genome-wide transcriptional profile of the reference strain ATCC 43300 MRSA in response to tannins has led to the finding that tannins induced significant modulation in essential ribosome pathways, which caused a reduction in the translation processes that lead to inhibition of protein synthesis and obviation of bacterial growth. These findings highlight the potential of tannins as new promising anti-MRSA agents in clinical application such as body wash and topical cream or ointments. � GERMS 2017.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.18683/germs.2017.1125
dc.identifier.epage192
dc.identifier.issn22482997
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85037578271
dc.identifier.spage186
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85037578271&doi=10.18683%2fgerms.2017.1125&partnerID=40&md5=aff0d57f3889fa937bf3ddf0b7dfaf83
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9617
dc.identifier.volume7
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherEuropean Academy of HIV/AIDS and Infectious Diseasesen_US
dc.relation.ispartofGERMS
dc.sourceScopus
dc.subjectAntimicrobial assaysen_US
dc.subjectMRSAen_US
dc.subjectRibosomal protein synthesisen_US
dc.subjectTanninsen_US
dc.titleDisruption of methicillin-resistant Staphylococcus aureus protein synthesis by tanninsen_US
dc.title.alternativeGERMSen_US
dc.typeArticleen_US
dspace.entity.typePublication

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