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Anti-biofilm and anti-adherence activities of sub fraction 18 of melastoma malabathricum towards streptococcus mutans

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dc.Conferencecode111523
dc.Conferencedate9 April 2014 through 11 April 2014
dc.Conferencename2014 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2014
dc.contributor.affiliationsFaculty of Dentistry
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorRohazila M.H.en_US
dc.contributor.authorNazlina I.en_US
dc.contributor.authorYaacob W.A.en_US
dc.date.accessioned2024-05-29T01:57:47Z
dc.date.available2024-05-29T01:57:47Z
dc.date.issued2014
dc.description.abstractA study was carried out to isolate and identify the active compounds from Melastoma malabathricum stem bark that exhibit anti-biofilm and anti-adherence activities against Streptococcus mutans. Purification of the active compounds from the stem bark extract was performed via silica gel chromatography to produce 12 fractions. Further fractionation of fraction 9 by high performance liquid chromatography (HPLC) produced 21 sub fractions. All the sub fractions were subjected to thin layer chromatography (TLC) bioautography as preliminary screening to determine anti bacterial activity. TLC-bioautography showed that sub fraction 18 (SF18) demonstrated large inhibited zone against S. mutans. Gas chromatography mass spectrometry (GCMS) was used to identify the active compounds in SF18. Fraction SF18 revealed 27 compounds such as hexanoic acid, 8-methyl-1-undecene, propanenitrile, and 1-decene. Anti-biofilm and anti-adherence activities were determined using crystal violet and glass surface assays respectively. The concentrations that produced 50% reduction in anti-biofilm and anti-adherence activities were 1.88 mg/ml and 3.75 mg/ml respectively. � 2014 AIP Publishing LLC.
dc.description.natureFinalen_US
dc.editorIbrahim Z.Maideen H.M.K.Ibrahim N.Karim N.H.A.Yusof T.Razak F.A.Maseran N.Khalid R.M.Ibrahim N.B.Sidek H.M.Noorani M.S.M.Simon N.en_US
dc.identifier.doi10.1063/1.4895261
dc.identifier.epage561
dc.identifier.isbn9780740000000
dc.identifier.issn0094243X
dc.identifier.scopus2-s2.0-85063832418
dc.identifier.spage557
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85063832418&doi=10.1063%2f1.4895261&partnerID=40&md5=8efdf1d6f50bdb80cb85f0283ed8d745
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9955
dc.identifier.volume1614
dc.languageEnglish
dc.language.isoen_US
dc.publisherAmerican Institute of Physics Inc.en_US
dc.relation.ispartofAIP Conference Proceedings
dc.sourceScopus
dc.subjectAnti-biofilm and anti-adherence activityen_US
dc.subjectMelastoma malabathricumen_US
dc.titleAnti-biofilm and anti-adherence activities of sub fraction 18 of melastoma malabathricum towards streptococcus mutans
dc.typeConference Paperen_US
dspace.entity.typePublication

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