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In-depth spectral characterization of antioxidative (1,3)-β-D-glucan from the mycelium of an identified tiger milk mushroom Lignosus rhinocerus strain ABI in a stirred-tank bioreactor

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dc.contributor.affiliationsGENIUS Insan College
dc.contributor.affiliationsNational Institutes of Biotechnology Malaysia (NIMB)
dc.contributor.affiliationsUniversity of Malaya (UM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorAhmad Usuldin S.R.en_US
dc.contributor.authorMahmud N.en_US
dc.contributor.authorIlham Z.en_US
dc.contributor.authorKhairul Ikram N.K.en_US
dc.contributor.authorAhmad R.en_US
dc.contributor.authorWan-Mohtar W.A.A.Q.I.en_US
dc.date.accessioned2024-05-29T02:04:40Z
dc.date.available2024-05-29T02:04:40Z
dc.date.issued2020
dc.description.abstractA rare Malaysian tiger milk mushroom Lignosus rhinocerus strain ABI (LRSA) was morphologically identified based on its pileus, stipe, and sclerotium. LRSA (515 bp) was sequenced and found to have 99% similar to L. rhinocerus strains CH31 and CH2. Phylogenetically, evolutionary distance (Knuc) and plasmid-matching software (ApE) for sequences of matching fungal species were used to verify that the isolate belonged to the L. rhinocerus species. The strain was cultured in a stirred-tank bioreactor and a mycelial β-glucan (G) was extracted for compound characterization. The structure of exopolysaccharide extract from mycelium of LRSA was studied using Fourier-transform infrared spectroscopy (FT-IR) and one-dimensional (1D) and two-dimensional (2D) Nuclear Magnetic Resonance (NMR). FT-IR spectroscopy showed that G exhibited a similar β-glycosidic structure to the standard (laminarin), and the presence of characteristic bands at 3277, 2919, 1638, 1545, 1400, 1078, and 896 cm−1 confirmed the similarities. 1H and 13C NMR, as well as 2D NMR: homonuclear correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), heteronuclear multiple quantum coherence (HMQC), and heteronuclear multiple bond coherence (HMBC) spectra, were used for structural elucidation of the β-glucan and confirmed the extracted material as (1,3)-β-D-glucan. In addition, the G compound exhibited antioxidant activities through total phenolic content (4.47 mg gallic acid equivalents/g), 2, 2-diphenyl-1-picrylhydrazyl (11.48 mg/mL), and ferric reducing antioxidant power (0.56 mg/mL) assays. These findings may facilitate the development of rare G production in a high-scale bioreactor using LRSA.en_US
dc.identifier.ArtNo101455
dc.identifier.doi10.1016/j.bcab.2019.101455
dc.identifier.issn18788181
dc.identifier.scopus2-s2.0-85075879880
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85075879880&doi=10.1016%2fj.bcab.2019.101455&partnerID=40&md5=e29d7ac975d7af7a04bcce11599c51fa
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10302
dc.identifier.volume23
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofBiocatalysis and Agricultural Biotechnologyen_US
dc.sourceScopus
dc.subject(1,3)-β -D-glucanen_US
dc.subject2D NMRen_US
dc.subjectAntioxidanten_US
dc.subjectBioreactor fermentationen_US
dc.subjectLignosus rhinocerusen_US
dc.subjectTiger milk mushroomen_US
dc.titleIn-depth spectral characterization of antioxidative (1,3)-β-D-glucan from the mycelium of an identified tiger milk mushroom Lignosus rhinocerus strain ABI in a stirred-tank bioreactoren_US
dc.typeArticleen_US
dspace.entity.typePublication

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