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Investigations for the possible use of a monoclonal antibody produced against Strongyloides ratti antigen as an immunodiagnostic reagent for active strongyloidiasis

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dc.FundingDetailsUniversiti Putra Malaysia: 04-02-12-2097RU Ministry of Science, Technology and Space: 02-01-04-SF1655 Ministry of Higher Education, Malaysia,�MOHE: 04-01-11-1067FR
dc.FundingDetailsThis work was supported, in parts, by grants from the Ministry of Higher Education Malaysia (04-01-11-1067FR), Ministry of Science, Technology and Innovation Malaysia (02-01-04-SF1655) and University Putra Malaysia (04-02-12-2097RU).
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUsmanu Danfodiyo University
dc.contributor.affiliationsUniversity of Anbar
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorMahmuda A.en_US
dc.contributor.authorBande F.en_US
dc.contributor.authorAbdulhaleem N.en_US
dc.contributor.authorAbd Majid R.en_US
dc.contributor.authorAwang Hamat R.en_US
dc.contributor.authorOmar Abdullah W.en_US
dc.contributor.authorUnyah Z.en_US
dc.date.accessioned2024-05-28T08:45:16Z
dc.date.available2024-05-28T08:45:16Z
dc.date.issued2018
dc.description.abstractBackground: Currently, most of the available serological diagnostic kits for strongyloidiasis are based on the use of the crude antigens of Strongyloides ratti, which are good, but with less sensitivity towards the infection. Hence, this study aimed to produce and evaluate monoclonal antibody for detecting soluble parasite antigen in animal sera. Methods: The study was conducted in the Department of Medical Microbiology and Parasitology, University Putra Malaysia in 2014-2017. Saline extract protein from the infective larvae of S. ratti was used to immunize BALB/c mice and subsequent fusion of the B-cells with myeloma cells (SP2/0) using 50% PEG. The hybridomas were cultured in HAT medium and cloned by limiting dilutions. Positive hybrids were screened by indirect ELISA. The ascites fluid from the antibody-secreting hybridoma was purified and the MAb was characterized by western-blots and evaluated in sandwich ELISA for reactivity against the homologous and heterologous antigens. Results: An IgG1 that recognizes a 30 and 34 kDa protein bands was obtained. The MAb was recognized by all S. ratti-related antigens and cross-reacted with only Toxocara canis antigens in both assays. The minimum antigen detection limit was found to be 5 ng/ml. All antibody-positive rat and dog sera evaluated have shown antigen-positive reactions in Sandwich-ELISA. Conclusion: The MAb produced, was able to detect antigens in strongyloidiasis and toxocariasis in animal models and may also be useful for the serological detection of active strongyloidiasis and visceral toxocariasis in human sera. � 2018, Tehran University of Medical Sciences (TUMS). All rights reserved.en_US
dc.description.natureFinalen_US
dc.identifier.epage214
dc.identifier.issn17357020
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85049132113
dc.identifier.spage204
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85049132113&partnerID=40&md5=0fdc5c037fbf262aa2fd9bf8625ff265
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9403
dc.identifier.volume13
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherTehran University of Medical Sciences (TUMS)en_US
dc.relation.ispartofIranian Journal of Parasitology
dc.sourceScopus
dc.subjectActive strongyloidiasisen_US
dc.subjectAntigenen_US
dc.subjectImmunodiagnostic reagenten_US
dc.subjectMonoclonal antibodyen_US
dc.subjectStrongyloides rattien_US
dc.subjectVisceral toxocariasisen_US
dc.titleInvestigations for the possible use of a monoclonal antibody produced against Strongyloides ratti antigen as an immunodiagnostic reagent for active strongyloidiasisen_US
dc.title.alternativeIran. J. Parasitol.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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