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Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

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dc.contributor.authorSugenendran Supramanien_US
dc.contributor.authorNur Ardiyana Rejaben_US
dc.contributor.authorZul Ilhamen_US
dc.contributor.authorRahayu Ahmaden_US
dc.contributor.authorPau-Loke Showen_US
dc.contributor.authorMohamad Faizal Ibrahimen_US
dc.contributor.authorWan Abd Al Qadr Imad Wan-Mohtaren_US
dc.date.accessioned2024-05-28T06:37:44Z
dc.date.available2024-05-28T06:37:44Z
dc.date.issued2023
dc.date.submitted2023-5-11
dc.descriptionVolume 11 Issue 3 Page (1-14)en_US
dc.description.abstractConventional stirred-tank bioreactor (STR) designs are optimised for cultures of bacteria but not fungal cultures; therefore, a new Air-L-Shaped Bioreactor (ALSB) was fabricated. The ALSB was designed to eliminate the wall growth and clumping of fungal mycelium in STRs. Ganoderma lucidum was used as a fungal model and its biomass and exopolysaccharide (EPS) production were maximised by optimising the agitation rate, glucose concentration, initial pH, and aeration via response surface methodology (RSM). The ALSB system generated 7.8 g/L of biomass (biomass optimised conditions: 110 rpm, 24 g/L glucose, pH 5.6, and 3 v/v of aeration) and 4.4 g/L of EPS (EPS optimised conditions: 90 rpm, 30 g/L glucose, pH 4, and 2.5 v/v of aeration). In combination, for both optimised conditions, biomass (7.9 g/L) and EPS (4.6 g/L) were produced at 110 rpm, 30 g/L glucose, pH 4, and 3 v/v of aeration with minimal wall growth. The data prove that the ALSB is a blueprint for efficient economical fungal cultivation.en_US
dc.identifier.doi10.3390/pr11030670
dc.identifier.epage14
dc.identifier.issn2227-9717
dc.identifier.issue2023
dc.identifier.other2282-17
dc.identifier.spage1
dc.identifier.urihttps://www.mdpi.com/2227-9717/11/3/670
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/7406
dc.identifier.volume11
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.ispartofProcessesen_US
dc.subjectGanoderma lucidum; smart bioreactor; bioprocess optimization; wall growth; exopolysaccharideen_US
dc.titlePerformance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)en_US
dc.typeArticleen_US
dspace.entity.typePublication

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