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In Vitro Activity, Stability and Molecular Characterization of Eight Potent Bacteriophages Infecting Carbapenem-resistant Klebsiella Pneumoniae

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dc.contributor.authorAbeer Ameen Baqeren_US
dc.contributor.authorKokxin Fangen_US
dc.contributor.authorNorfarhan Mohd-Assaaden_US
dc.contributor.authorSiti Noor Adnalizawati Adnanen_US
dc.contributor.authorNorefrina Shafinaz Md Noren_US
dc.date.accessioned2024-05-29T02:25:47Z
dc.date.available2024-05-29T02:25:47Z
dc.date.issued2022
dc.date.submitted2023-1-26
dc.descriptionVolume:15 Issue:1en_US
dc.description.abstractBackground: Members of the genus Klebsiella are among the leading microbial pathogens associated with nosocomial infection. The increased incidence of antimicrobial resistance in these species has propelled the need for alternate/combination therapeutic regimens to aid clinical treatment, including bacteriophage therapy. Bacteriophages are considered very safe and effective in treating bacterial infections. In this study, we characterize eight lytic bacteriophages that were previously isolated by our team against carbapenem-resistant Klebsiella pneumoniae. Methods: The one-step-growth curves, stability and lytic ability of eight bacteriophages were characterized. Restriction fragment length polymorphism (RFLP), random amplification of polymorphic DNA (RAPD) typing analysis and protein profiling were used to characterize the microbes at the molecular level. Phylogenetic trees of four important proteins were constructed for the two selected bacteriophages. Results and conclusions: All eight bacteriophages showed high efficiency for reducing bacterial concentration with high stability under different physical and chemical conditions. We found four major protein bands out of at least ten 15–190 KDa bands that were clearly separated by SDS-PAGE, which were assumed to be the major head and tail proteins. The genomes were found to be dsDNA, with sizes of approximately 36–87 Kb. All bacteriophages reduced the optical density of the planktonic K. pneumoniae abruptly, indicating great potential to reduce K. pneumoniae infection. In this study, we have found that tail fiber protein can further distinguished closely related bacteriophages. The characterised bacteriophages showed promising potential as candidates against carbapenem-resistant Klebsiella pneumoniae via bacteriophage therapy.en_US
dc.identifier.citationBaqer, A.A.; Fang, K.; Mohd-Assaad, N.; Adnan, S.N.A.; Md Nor, N.S. In Vitro Activity, Stability and Molecular Characterization of Eight Potent Bacteriophages Infecting Carbapenem-Resistant Klebsiella pneumoniae. Viruses 2023, 15, 117. https://doi.org/10.3390/v15010117en_US
dc.identifier.doi10.3390/v15010117
dc.identifier.epage21
dc.identifier.issn1999-4915
dc.identifier.issue1
dc.identifier.spage1
dc.identifier.urihttps://www.mdpi.com/1999-4915/15/1/117
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85147045387&origin=resultslist&sort=plf-f&src=s&sid=ff7b2cafb526bfeca0fe6ae95dfa2def&sot=b&sdt=b&s=TITLE-ABS-KEY%28In+Vitro+Activity%2C+Stability+And+Molecular+Characterization+Of+Eight+Potent+Bacteriophages+Infecting+Carbapenem-resistant+Klebsiella+Pneumoniae%29&sl=113&sessionSearchId=ff7b2cafb526bfeca0fe6ae95dfa2def
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10510
dc.identifier.volume15
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.ispartofVirusesen_US
dc.subjectbacteriophage; Klebsiella pneumoniae; phage therapy; antibiotics alternativeen_US
dc.titleIn Vitro Activity, Stability and Molecular Characterization of Eight Potent Bacteriophages Infecting Carbapenem-resistant Klebsiella Pneumoniaeen_US
dc.typeArticleen_US
dspace.entity.typePublication

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