Publication: A new ultra violet-visible spectrophotometric method for quantitative determination of acrylamide via hydrolysis process
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Date
2019
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Publisher
Malaysian Society of Analytical Sciences
Abstract
This paper reported the results for quantitative determination of acrylamide via hydrolysis process using ultra violet-visible (UV-Vis) spectrophotometric method. This quantitative determination started with hydrolysing acrylamide in a strong basic condition to yield ammonia and acid salt. The optimum conditions of hydrolysis (concentration of the base used and time for hydrolysis) were also determined. From this study, the optimum conditions to hydrolyse acrylamide were achieved using 6.0 M of sodium hydroxide (NaOH) for 10 minutes. The hydrolysis process was characterised by monitoring the ammonia produced using Nessler’s reagent through the formation of yellow colouration in the presence of ammonia. After the optimisation of hydrolysis process, the characterisation of all parameters including concentration of Nessler’s reagent used, reproducibility, dynamic range, and interference ions were studied. Linear dynamic ranges from 0-10 ppm acrylamide with limit of detection (LOD) of 0.074 ppm were obtained when 3.0 mM Nessler’s reagent was used. The relative standard deviations (RSD) for reproducibility were 2.8-3.3%. No significant interference from cations such as Na + , K + , Ca 2+ during quantitative analysis of acrylamide, but ions such as Fe 3+ and NH 3 affected the analysis.
Description
Malaysian Journal of Analytical Sciences,
Vol 23 No 1 (2019): 14 - 22
Keywords
Acrylamide, Hydrolysis, Nessler's reagent