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Transcriptome analysis of methicillin-resistant Staphylococcus aureus in response to stigmasterol and lupeol

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dc.Chemicals/CASlupeol, 545-47-1; stigmasterol, 83-48-7; meticillin, 132-92-3, 38882-79-0, 61-32-5; Anti-Bacterial Agents; Drug Combinations; lupeol; Methicillin; Pentacyclic Triterpenes; RNA, Ribosomal, 16S; Stigmasterol
dc.citedby4
dc.contributor.affiliationsFaculty of Dentistry
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.authorAdnan S.-N.-A.en_US
dc.contributor.authorIbrahim N.en_US
dc.contributor.authorYaacob W.A.en_US
dc.date.accessioned2024-05-28T08:46:10Z
dc.date.available2024-05-28T08:46:10Z
dc.date.issued2017
dc.description.abstractObjectives Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen with multiple antibiotic resistance that causes morbidity and mortality worldwide. Multidrug-resistant (MDR) MRSA with increased resistance to currently available antibiotics has challenged the world to develop new therapeutic agents. Stigmasterol and lupeol, from the plant Phyllanthus columnaris, exhibit antibacterial activities against MRSA. The aim of this study was to utilise next-generation sequencing (NGS) to provide further insight into the novel transcriptional response of MRSA exposed to stigmasterol and lupeol. Methods Time�kill analysis of one MRSA reference strain (ATCC 43300) and three clinical isolates (WM3, BM1 and KJ7) for both compounds was first performed to provide the bacteriostatic/bactericidal profile. Then, MRSA ATCC 43300 strain treated with both compounds was interrogated by NGS. Results Both stigmasterol and lupeol possessed bacteriostatic properties against all MRSA tested; however, lupeol exhibited both bacteriostatic and bactericidal properties within the same minimum inhibitory concentration and minimum bactericidal concentration values against BM1 (12.5 mg/mL). Transcriptome profiling of MRSA ATCC 43300 revealed significant modulation of gene expression with multiple desirable targets by both compounds, which caused a reduction in the translation processes leading to inhibition of protein synthesis and prevention of bacterial growth. Conclusions This study highlights the potential of both stigmasterol and lupeol as new promising anti-MRSA agents. � 2016 International Society for Chemotherapy of Infection and Canceren_US
dc.description.natureFinalen_US
dc.identifier.doi10.1016/j.jgar.2016.10.006
dc.identifier.epage54
dc.identifier.issn22137165
dc.identifier.pmid27992774
dc.identifier.scopus2-s2.0-85006371054
dc.identifier.spage48
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85006371054&doi=10.1016%2fj.jgar.2016.10.006&partnerID=40&md5=6cd32aec8345c7d96caee562ecc16b2e
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9428
dc.identifier.volume8
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofJournal of Global Antimicrobial Resistance
dc.sourceScopus
dc.subjectLupeolen_US
dc.subjectMRSAen_US
dc.subjectProtein synthesisen_US
dc.subjectStigmasterolen_US
dc.subjectTime-kill analysisen_US
dc.subjectTranscriptome profilingen_US
dc.titleTranscriptome analysis of methicillin-resistant Staphylococcus aureus in response to stigmasterol and lupeolen_US
dc.title.alternativeJ. Global Antimicrob. Resist.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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