Publication:
Parkinson’s Disease Model In Zebrafish using Intraperitoneal MPTP Injection

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dc.contributor.authorNoor Azzizah Omaren_US
dc.contributor.authorJaya Kumaren_US
dc.contributor.authorSeong Lin Teohen_US
dc.date.accessioned2024-05-29T02:28:13Z
dc.date.available2024-05-29T02:28:13Z
dc.date.issued2023
dc.date.submitted2023-10-4
dc.description.abstractIntroduction: Parkinson’s disease (PD) is the second most common neurodegenerative disease that severely affects the quality of life of patients and their family members. Exposure to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been shown to reflect behavioral, molecular, and proteomic features of PD. This study aimed to assess the protocol for inducing PD following MPTP injection in adult zebrafish. Methods: Fish were injected with 100 μg/g of MPTP intraperitoneally once or twice and then assessed on days 1 to 30 post-injection. Results: Between one-time and two-time injections, there was no significant difference in most locomotor parameters, expressions of tyrosine hydroxylase-2 (th2) and dopamine transporter (dat) genes, and dopaminergic neurons (tyrosine hydroxylase positive, TH+ cells) counts. However, caspase-3 levels significantly differed between one- and two-time injections on the day 1 assessment. Discussion: Over a 30-day period, the parameters showed significant differences in swimming speed, total distance traveled, tyrosine hydroxylase-1 (th1) and dat gene expressions, caspase-3 and glutathione protein levels, and TH+ cell counts. Days 3 and 5 showed the most changes compared to the control. In conclusion, a one-time injection of MPTP with delayed assessment on days 3 to 5 is a good PD model for animal studies.en_US
dc.identifier.citationOmar NA, Kumar J and Teoh SL (2023) Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection. Front. Neurosci. 17:1236049. doi: 10.3389/fnins.2023.1236049en_US
dc.identifier.doi10.3389/fnins.2023.1236049
dc.identifier.epage17
dc.identifier.issn1662-4548
dc.identifier.issue2023
dc.identifier.spage1
dc.identifier.urihttps://www.frontiersin.org/articles/10.3389/fnins.2023.1236049/full
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85170258364&origin=resultslist&sort=plf-f&src=s&sid=69810be05facc93c4e9850e6fa9f3e49&sot=b&sdt=b&s=TITLE-ABS-KEY%28Parkinson%E2%80%99s+disease+model+in+zebrafish+using+intraperitoneal+MPTP+injection%29&sl=46&sessionSearchId=69810be05facc93c4e9850e6fa9f3e49
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10747
dc.identifier.volume17
dc.language.isoen_USen_US
dc.publisherFrontiersinen_US
dc.relation.ispartofFrontiers in Neuroscienceen_US
dc.subjectneurodegenerative disease, Danio rerio, tyrosine hydroxylase, dopaminergic neuron, neurotoxinen_US
dc.titleParkinson’s Disease Model In Zebrafish using Intraperitoneal MPTP Injectionen_US
dc.typeArticleen_US
dspace.entity.typePublication

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