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Effects of keratinocyte growth factor on skin epithelial differentiation of human amnion epithelial cells

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dc.Chemicals/CASinvolucrin, 60108-77-2; keratinocyte growth factor, 126469-10-1; nestin, 146315-66-4; Fibroblast Growth Factor 7; Keratin-14; Keratin-18
dc.FundingDetailsUniversiti Kebangsaan Malaysia Ministry of Science, Technology and Space: 02-01-02-SF0289
dc.FundingDetailsThis study was funded by the Ministry of Science, Technology and Innovations , Grant number: 02-01-02-SF0289 . Simat Siti Fatimah is our PhD student in Universiti Kebangsaan Malaysia and is a National Science Fellowship scholar of Ministry of Science, Technology and Innovations.
dc.MolecularSequenceNumbersGENBANK: NM_000224, NM_000526, NM_002007, NM_002046, NM_002276, NM_002701, NM_003106, NM_003508, NM_003722, NM_004334, NM_004763, NM_004827, NM_005547, NM_006121, NM_006617, NM_024865, NM_057088, NM_174900
dc.citedby5
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorFatimah S.S.en_US
dc.contributor.authorTan G.C.en_US
dc.contributor.authorChua K.en_US
dc.contributor.authorTan A.E.en_US
dc.contributor.authorNur Azurah A.G.en_US
dc.contributor.authorHayati A.R.en_US
dc.date.accessioned2024-05-29T01:56:06Z
dc.date.available2024-05-29T01:56:06Z
dc.date.issued2013
dc.description.abstractThe aim of the present study was to determine the effects of KGF on the differentiation of cultured human amnion epithelial cells (HAECs) towards skin keratinocyte. HAECs at passage 1 were cultured in medium HAM's F12: Dulbecco's Modified Eagles Medium (1:1) supplemented with different concentrations of KGF (0, 5, 10, 20, 30 and 50 ng/ml KGF). Dose-response of KGF on HAECs was determined by morphological assessment; growth kinetic evaluation; immunocytochemical analysis; stemness and epithelial gene expression quantification with two step real time RT-PCR. KGF promotes the proliferation of HAECs with maximal effect observed at 10 ng/ml KGF. However, KGF decreased the stemness genes expression: Oct-3/4, Sox-2, Nanog3, Rex-1, FGF-4, FZD-9 and BST-1. KGF also down-regulates epithelial genes expression: CK3, CK18, CK19, Integrin-?1, p63 and involucrin in cultured HAECs. No significant difference on the gene expression was detected for each Nestin, ABCG-2, CK1 and CK14 in KGF-treated HAECs. Immunocytochemical analysis for both control and KGF-treated HAECs demonstrated positive staining against CK14 and CK18 but negative staining against involucrin. The results suggested that KGF stimulates an early differentiation of HAECs towards epidermal cells. Differentiation of KGF-treated HAECs to corneal lineage is unfavourable. Therefore, further studies are needed to elucidate the roles of KGF in the differentiation of HAECs towards skin keratinocytes. � 2012 Elsevier Ltd and ISBI. All rights reserved.en_US
dc.description.natureFinalen_US
dc.identifier.CODENBURND
dc.identifier.doi10.1016/j.burns.2012.10.019
dc.identifier.epage915
dc.identifier.issn3054179
dc.identifier.issue5
dc.identifier.pmid23273814
dc.identifier.scopus2-s2.0-84879014238
dc.identifier.spage905
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84879014238&doi=10.1016%2fj.burns.2012.10.019&partnerID=40&md5=66070c6270dae9974d5a9cdd5478bf38
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9796
dc.identifier.volume39
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofBurns
dc.sourceScopus
dc.subjectAmnion cellsen_US
dc.subjectDifferentiationen_US
dc.subjectEpithelialen_US
dc.subjectKeratinen_US
dc.subjectKeratinocyte growth factoren_US
dc.subjectSkinen_US
dc.subjectStemnessen_US
dc.titleEffects of keratinocyte growth factor on skin epithelial differentiation of human amnion epithelial cellsen_US
dc.title.alternativeBurnsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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