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Development and validation of a RP-HPLC method for the quantification of omeprazole in pharmaceutical dosage form

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dc.contributor.authorRuhul Kayeshen_US
dc.contributor.authorMZ Sultanen_US
dc.contributor.authorAsma Abdul Rahmanen_US
dc.contributor.authorMG Uddinen_US
dc.contributor.authorF Aktaren_US
dc.contributor.authorMA Rashiden_US
dc.date.accessioned2024-05-27T14:36:16Z
dc.date.available2024-05-27T14:36:16Z
dc.date.issued2013
dc.description.abstractA rapid and highly sensitive reversed phase high performance liquid chromatographic method has been developed for quantitative estimation of omeprazole in pharmaceutical preparations. The method has been validated according to FDA and USP guidelines with respect to accuracy, precision, specificity and linearity. The method was developed by using a gradient condition of mobile phase comprising 90% aqueous acetonitrile to 100% acetonitrile for 10 minutes at a flow rate of 0.7 mL/min over C-18 (ODS, 250 x 4.6 mm) column at ambient temperature. More than 97% recovery demonstrated the accuracy of the protocol. Intra-day and inter-day precision studies of the new method were less than the maximum allowable limit (RSD%� 2.0 according to FDA). The method showed linear response with correlation coefficient (r 2) value of 0.998. Therefore, it was found to be accurate, reproducible, sensitive and less time consuming and can be successfully applied for the assay of omeprazole formulations.
dc.identifier.doi10.3329/jsr.v5i2.12779
dc.identifier.epage342
dc.identifier.issn2070-0237
dc.identifier.issue2
dc.identifier.spage335
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/3408
dc.identifier.volume5
dc.languageEnglish
dc.publisherJSR Publicationsen_US
dc.relation.ispartofJournal of Scientific Research
dc.subjectHPLC; Method development; Gradient condition; Validation; Omeprazole
dc.titleDevelopment and validation of a RP-HPLC method for the quantification of omeprazole in pharmaceutical dosage formen_US
dspace.entity.typePublication

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