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Microscopy-based techniques: A reliable method for detecting Blastocystis sp. Infection

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dc.FundingDetailsFF-2014-219 Universiti Kebangsaan Malaysia: DLP-2014-013
dc.FundingDetailsWe gratefully acknowledge the Ministry of Rural and Regional Development Malaysia to permit this research to be conducted and Meteorological Department of Pahang for their technical support. This study was supported partly by the UKMMC Fundamental Research Grant (FF-2014-219) and UKM Publication Enhancement Grant (DLP-2014-013).
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM) Medical Centre
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Teknologi MARA (UiTM)
dc.contributor.affiliationsKolej Universiti INSANIAH
dc.contributor.affiliationsUniversiti Tunku Abdul Rahman (UTAR)
dc.contributor.authorNoradilah S.A.en_US
dc.contributor.authorMoktar N.en_US
dc.contributor.authorAnuar T.S.en_US
dc.contributor.authorLee I.L.en_US
dc.contributor.authorSalleh F.M.en_US
dc.contributor.authorManap S.N.A.A.en_US
dc.contributor.authorMohtar N.S.H.M.en_US
dc.contributor.authorAzrul S.M.en_US
dc.contributor.authorAbdullah W.O.en_US
dc.contributor.authorNordin A.en_US
dc.contributor.authorAbdullah S.R.en_US
dc.date.accessioned2024-05-28T08:28:48Z
dc.date.available2024-05-28T08:28:48Z
dc.date.issued2017
dc.description.abstractMicroscopy technique is widely used in the detection of Blastocystis sp. infection. This study was performed to compare two microscopy-based techniques; in vitro cultivation of faecal specimens in Jones' medium (IVC) followed by Wheatley Trichrome staining and fixed-PVA faecal specimens (PVA) followed by Wheatley Trichrome staining with single-round polymerase chain reaction (PCR) as the reference technique. Examination was performed on 466 faecal samples obtained from Orang Asli community in Pahang, Malaysia. Detection rate of Blastocystis sp. was highest in IVC (35.6%) followed by PVA (20.0%). Single-round PCR detected 41.0% of the faecal specimens. The sensitivity and specificity of PVA and IVC compared to the reference technique were 75.3% (95% CI: 65.2-83.6) and 68.5% (CI: 63.7-73.3), and 88.6% (CI: 82.7-93.0) and 86.3% (CI: 81.9-90.0) respectively. The agreement between the reference technique and PVA showed fair statistically significant agreement by Cohen Kappa statistics of (K=0.318, p<0.001), meanwhile substantial statistically significant agreement was observed between PCR and IVC by Cohen Kappa (K=0.727, p<0.001). Therefore, in vitro cultivation in Jones' medium followed by Wheatley Trichrome staining of faecal specimens should be used as a screening technique in the detection Blastocystis sp. infections. � 2017 Oriental Scientific Publishing Company. Al rights reserved.en_US
dc.description.natureFinalen_US
dc.identifier.citationSylwanVolume 161, Issue 6, Pages 149 - 1532017en_US
dc.identifier.epage153
dc.identifier.issn397660
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85047445870
dc.identifier.spage149
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85047445870&partnerID=40&md5=6be0b8f3e83b31dd795a1537670cb9cb
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8873
dc.identifier.volume161
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherPolish Forest Societyen_US
dc.relation.ispartofSylwanen_US
dc.sourceScopus
dc.titleMicroscopy-based techniques: A reliable method for detecting Blastocystis sp. Infectionen_US
dc.typeArticleen_US
dspace.entity.typePublication

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