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The effectiveness of gentamicin against acanthamoeba cysts in vitro

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dc.citedby1
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.authorNoradilah S.A.en_US
dc.contributor.authorMohamed Kamel A.G.en_US
dc.contributor.authorAnisah N.en_US
dc.contributor.authorNoraina A.R.en_US
dc.contributor.authorYusof S.en_US
dc.date.accessioned2024-05-29T01:59:10Z
dc.date.available2024-05-29T01:59:10Z
dc.date.issued2012
dc.description.abstractAcanthamoeba is a free-living protozoa which causes serious ocular problem. Acanthamoeba keratitis is becoming more prevalent amongst contact lens wearers and it can cause loss of vision and blindness if not treated properly. The objective of this research is to determine the effectiveness of gentamicin against six Acanthamoeba spp. isolates, of which three were clinical isolates (HS 6, HKL 95, HTH 73) and three environmental isolates (SMAL 7, SMAL 8, TTT 9). Cyst suspension from the chosen isolates were exposed to gentamicin. After 48 hours of incubation at temperature of 30�C and 37�C, each mixture was filtered and filtration membrane was put onto non-nutrient agar laid with Escherichia coli. The agar plates were incubated for three days at 30oC and 37oC and the plates were examined daily until day 14 to look for the presence of Acanthamoeba trophozoites under inverted microscope. The presence of trophozoites indicated the ineffectiveness of gentamicin. Gentamicin was found to be effective against Acanthamoeba cysts from all the test strains at both incubation temperatures. The minimum cysticidal concentration (MCC) mean value of gentamicin was 0.193 mg/mL at 30oC and 0.229 mg/mL at 37oC. So, we concluded that gentamicin has cysticidal potential towards Acanthamoeba.en_US
dc.description.natureFinalen_US
dc.identifier.epage54
dc.identifier.issn16758544
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84873906244
dc.identifier.spage51
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84873906244&partnerID=40&md5=e2d14f04bc34ccebe8dd3ccaaff4315b
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10044
dc.identifier.volume8
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofMalaysian Journal of Medicine and Health Sciencesen_US
dc.sourceScopus
dc.subjectAcanthamoebaen_US
dc.subjectGentamicinen_US
dc.subjectSensitivityen_US
dc.titleThe effectiveness of gentamicin against acanthamoeba cysts in vitroen_US
dc.typeArticleen_US
dspace.entity.typePublication

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