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Ferrocene Mediated Amperometric Biosensor For L-Glutamate Based On L-Glutamate Oxidase Immobilized In A Photocurable Methacrylic Film

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dc.contributor.authorNoor Zuhartini Md Muslimen_US
dc.contributor.authorMusa Ahmaden_US
dc.date.accessioned2024-05-29T02:28:09Z
dc.date.available2024-05-29T02:28:09Z
dc.date.issued2022
dc.date.submitted2023-2-4
dc.descriptionVolume: 26 No: 6 (page: 1260 - 1273)en_US
dc.description.abstractL-glutamate is widely used as a flavour enhancer in various foodstuffs and seasoning in the form of monosodium glutamate (MSG). MSG has been linked to neurotoxic effects, metabolic disorders, headache, numbness and palpitation. In this work, an amperometric L-glutamate biosensor based on photocurable poly(2-hydroxylethyl methacrylate)-containing ferrocene film for the determination of L-glutamate in food samples is described. The sensor is fabricated based on simultaneous immobilization of both L-glutamate oxidase and ferrocene as a mediator during the deposition of poly(2-hydroxylethyl methacrylate) film via photocuring. Ferrocene was used to shuttle the electrons directly between the reduced enzyme and the electrode. From electrochemical studies, a linear response towards L-glutamate in the concentration range of 10-30 mM was obtained at applied potential of +0.25 V with the detection limit of 7.7 mM. The storage stability of the biosensor is up to 4 months under storage condition of 4 oC in refrigerator. The performance of the biosensor was applied to the determination of L-glutamate in food stocks from local supermarkets. Results from amperometric L-glutamate biosensor were further validated with HPLC method.en_US
dc.identifier.epage1273
dc.identifier.issn1394-2506
dc.identifier.issue6
dc.identifier.spage1260
dc.identifier.urihttps://mjas.analis.com.my/mjas/v26_n6/pdf/Muslim_26_6_10.pdf
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85144919414&origin=resultslist&sort=plf-f&src=s&sid=3f1868111a303d5a05d758a34d75371e&sot=b&sdt=b&s=TITLE-ABS-KEY%28Ferrocene+Mediated+Amperometric+Biosensor+For+L-Glutamate+Based+On+L-Glutamate+Oxidase+Immobilized+In+A+Photocurable+Methacrylic+Film%29&sl=151&sessionSearchId=3f1868111a303d5a05d758a34d75371e
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10743
dc.identifier.volume26
dc.language.isoen_USen_US
dc.publisherMalaysian Analytical Sciences Society (ANALIS)en_US
dc.relation.ispartofMalaysian Journal of Analytical Sciences (MJAS)en_US
dc.subjectphotocurable, methacrylate polymers, ferrocene, L-glutamate biosensoren_US
dc.titleFerrocene Mediated Amperometric Biosensor For L-Glutamate Based On L-Glutamate Oxidase Immobilized In A Photocurable Methacrylic Filmen_US
dc.title.alternative(Biosensor Amperometrik L-Glutamat berperantara Ferosena berasaskan L-Glutamat Oksida Terpegun dalam Fotosalutan Filem Metakriliken_US
dc.typeArticleen_US
dspace.entity.typePublication

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