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Acrylamide Optical Sensor Based on Hydrolysis Using Bacillus sp Strain ZK34 Containing Amidase Properties

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dc.FundingDetailsUniversiti Kebangsaan Malaysia
dc.FundingDetailsWe are gratefully acknowledged the financial support from Universiti Kebangsaan Malaysia via grant No. DIP-2014-016 and DPP-2015-064 and Ministry of Education to Universiti Sains Islam Malaysia (USIM) through research grants USIM/FRGS-FST-5-50311. We would also like to acknowledge the Ministry of Higher Education (MOHE), Malaysia for the MyBrain 15 scholarship to Yee-May Chong. Special thanks to UPM for bacterial strain and facilities provided.
dc.citedby1
dc.contributor.affiliationsFaculty of Science and Technology
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.authorChong Y.-M.
dc.contributor.authorAhmad M.
dc.contributor.authorHeng L.Y.
dc.contributor.authorKusnin N.
dc.contributor.authorShukor M.Y.A.
dc.date.accessioned2024-05-28T08:29:20Z
dc.date.available2024-05-28T08:29:20Z
dc.date.issued2017
dc.description.abstractIn this work, a new optical screening method for acrylamide was developed. Bacterial Bacillus sp. strain ZK 34 was used to hydrolyse acrylamide to the corresponding acid and ammonia. Nessler's reagent was used to detect the produced ammonia and the yellow complex formed was treated as signal. Bacterial pellet was immobilised in the alginate membrane. The optimum composition of alginate used is 2%. The mass ratio of alginate:bacterial of 1:0.5 gave the optimum respond. Optimum concentration for NaOH and Nessler's reagent were 0.075 M and 2.5 mM, respectively. The yellow complex of mercury (II) amido-iodine formed was directly proportional to the concentrations of acrylamide up to 50.00 ppm with the limit of detection of 1.30 ppm. This sensor shows a good reproducibility which the relatives standard deviation (RSD) values from 3.17-6.15%. Therefore, the detection of acrylamide based on the amidase hydrolysis is suitable for screening this carcinogen compound.
dc.description.natureFinal
dc.identifier.doi10.17576/jsm-2017-4609-26
dc.identifier.epage1563
dc.identifier.issn1266039
dc.identifier.issue9
dc.identifier.scopus2-s2.0-85038425275
dc.identifier.scopusWOS:000417356900026
dc.identifier.spage1557
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85038425275&doi=10.17576%2fjsm-2017-4609-26&partnerID=40&md5=2856b059095b8d6320a278773f9eb3b0
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8899
dc.identifier.volume46
dc.languageEnglish
dc.language.isoen_US
dc.publisherPenerbit Universiti Kebangsaan Malaysia
dc.relation.ispartofOpen Access
dc.relation.ispartofSains Malaysiana
dc.sourceScopus
dc.subjectAcrylamide
dc.subjectAmidase
dc.subjectNessler's reagent
dc.subjectOptical detection
dc.titleAcrylamide Optical Sensor Based on Hydrolysis Using Bacillus sp Strain ZK34 Containing Amidase Properties
dc.title.alternativeSains Malays.
dc.typeArticle
dspace.entity.typePublication

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