Extraction Techniques for Analyzing Citrus Hystrix and Lagenaria Siceraria Compounds for Gum Disease Via Gas Chromatography Mass Spectrometry

In this era of globalisation, many individuals who suffer from allergies or sensitivities to industrial medications and synthetic chemicals often look for organic and environmentally friendly alternatives. This trend reflects a growing preference for sustainable and eco-conscious choices in personal and healthcare products. To tackle this issue, we have identified bioactive compounds in Lagenaria siceraria and citrus hystrix leaves to showcase their effectiveness and compatibility for gum disease. These plants not only provide a safer and more natural option for consumers but also offer an alternative source of chemical components required by pharmacies and medical professionals for related products. To analyze the diversity of bioactive compounds in citrus hystrix leaves and Lagenaria siceraria, this study utilizes Gas Chromatography-Mass Spectrometry (GCMS). The samples were dried at 60°C for 24 hours and then mashed into a powder. The powder was weighed and mixed with a 4:1 hexane and ethyl acetate solvent in a ratio of 10 mL of solvent per gram of sample. The mixture was covered and left for 24 hours, then filtered to separate the solid residue from the liquid. The liquid was placed in a rotary evaporator set at 76°C and 264 ATM to remove the solvent, leaving behind the extracted oil. This extraction method will be employed to prepare samples for subsequent analysis using Gas Chromatography-Mass Spectrometry (GC-MS), ensuring accurate and detailed examination of the compounds present.