Publication:
Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

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dc.contributor.authorSabullah, MKen_US
dc.contributor.authorAbd Shukor, MYen_US
dc.contributor.authorShamaan, NAen_US
dc.contributor.authorKhalid, Aen_US
dc.contributor.authorGanzau, AJen_US
dc.contributor.authorSulaiman, MRen_US
dc.contributor.authorJirangon, Hen_US
dc.contributor.authorAhmad, SAen_US
dc.date.accessioned2024-05-29T02:49:39Z
dc.date.available2024-05-29T02:49:39Z
dc.date.issued2015
dc.description.abstractThe purification of a soluble cholinesterase (ChE) from Puntius javanicus liver using affinity chromatography was studied. Affinity matrix was synthesised through the cooling system of ligands procainamide to epoxy-activated Sephacryl 6B and purification process was performed using calibrated flow rate at 0.2 mL/min. Non-denaturing electrophoresis condition was employed and the single band native form of ChE was detected at 66.267 kDa after being stained with commasie brilliant blue. ChE detection was performed using gel filtration; ZORBAX column attached to the HPLC with the flow rate of 1 mL/min. Only a single peak was detected at the retention time of 3.720. From the assay evaluation, the final purified ChE procedure displayed the highest sensitivity of detecting the anticholinesterase namely mercury, copper, malaoxon and carbofuran compared to the impure ChE and the results were further discussed in detail to the potential application of ChE from P. javanicus as a biomarker for those toxicants. (C) 2015 Friends Science Publishers
dc.identifier.doi10.17957/IJAB/15.0012
dc.identifier.epage1030
dc.identifier.isbn1814-9596
dc.identifier.issn1560-8530
dc.identifier.issue5
dc.identifier.scopusWOS:000361559200023
dc.identifier.spage1025
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10898
dc.identifier.volume17
dc.languageEnglish
dc.language.isoen_US
dc.publisherFriends Science PUBLen_US
dc.relation.ispartofInternational Journal Of Agriculture And Biology
dc.sourceWeb Of Science (ISI)
dc.subjectPurificationen_US
dc.subjectCholinesteraseen_US
dc.subjectPuntius Javanicusen_US
dc.subjectAnticholinesteraseen_US
dc.titlePurification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus
dc.typeArticleen_US
dspace.entity.typePublication

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