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The improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)

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dc.Chemicals/CASdiethylstilbestrol, 30498-85-2, 56-53-1
dc.FundingDetailsUniversiti Putra Malaysia: 04-01-09-0714RU
dc.FundingDetailsThis work was supported by Research University Grant Scheme (RUGS) of Universiti Putra Malaysia ( 04-01-09-0714RU ).
dc.citedby2
dc.contributor.affiliationsFaculty of Dentistry
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorZulfahmi S.en_US
dc.contributor.authorYazan L.S.en_US
dc.contributor.authorIthnin H.en_US
dc.contributor.authorArmania N.en_US
dc.date.accessioned2024-05-29T01:55:11Z
dc.date.available2024-05-29T01:55:11Z
dc.date.issued2013
dc.description.abstractCervical cancer is the most common gynecological cancer and one of the major causes of female cancer-related death worldwide particularly in developing countries. Thus far, there are a few in vivo models have been developed in investigating this type of cancer. In this study, we induced cervical cancer in Balb/c mice by exploiting the carcinogenic property of diestylstilbestrol (DES). The Balb/c pregnant mice were given subcutaneous (SC) injection of 67 ?g/kg body weight of DES on GD 13, and the mice gave birth approximately at gestation day 19-22. Female offspring were reared and the body weight was recorded once weekly. The female offspring were sacrificed at age of 5 months. Upon termination, blood was collected in a plain tube via cardiac puncture and the reproductive tracts were collected and weighed. The reproductive tract sections were stained using H&E for observation of pathological changes. The progression of disease state was monitored by measuring the level of serum interleukin (IL-6) using the Mouse IL-6 ELISA Assay Kit (BD OptEIA�, USA). All parameters were compared with Not-induced group. The outcome of this study demonstrated a significant difference in body weight gain, reproductive organ weight, diameter of cervix and the level of serum IL-6 in the Induced group as compared to the Not-induced group (P< 0.05). Histopathological findings revealed the presence of adenosis only in the Induced group. It shows that DES could be employed as an agent to induce cervical carcinogenesis in animal model. In addition to that, new potential anti-cancer agents from various sources could be further evaluated using this technique. � 2013.en_US
dc.description.natureFinalen_US
dc.identifier.CODENETPAE
dc.identifier.doi10.1016/j.etp.2013.04.004
dc.identifier.epage1089
dc.identifier.issn9402993
dc.identifier.issue44050
dc.identifier.pmid23726752
dc.identifier.scopus2-s2.0-84885863378
dc.identifier.spage1083
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84885863378&doi=10.1016%2fj.etp.2013.04.004&partnerID=40&md5=aadad39a6b139fc9d3669547056b71c7
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9651
dc.identifier.volume65
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofExperimental and Toxicologic Pathology
dc.sourceScopus
dc.subjectAdenosisen_US
dc.subjectBalb/c miceen_US
dc.subjectCarcinogenesisen_US
dc.subjectDiethylstilbestrolen_US
dc.subjectIn vivo modelen_US
dc.subjectInterleukin-6en_US
dc.titleThe improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)en_US
dc.title.alternativeExp. Toxicol. Pathol.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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