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Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation

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dc.contributor.authorFariha, MMNen_US
dc.contributor.authorChua, KHen_US
dc.contributor.authorTan, GCen_US
dc.contributor.authorLim, YHen_US
dc.contributor.authorHayati, ARen_US
dc.date.accessioned2024-05-29T03:26:52Z
dc.date.available2024-05-29T03:26:52Z
dc.date.issued2013
dc.description.abstractHuman chorion-derived stem cells (hCDSC) were previously shown to demonstrate multipotent properties with promising angiogenic characteristics in monolayer-cell culture system. In our study, we investigated the angiogenic capability of hCDSC in 3-dimensional (3D) in vitro and in vivo angiogenic models for the purpose of future application in the treatment of ischaemic diseases. Human CDSC were evaluated for angiogenic and endogenic genes expressions by quantitative PCR. Growth factors secretions were quantified using ELISA. In vitro and in vivo vascular formations were evaluated by histological analysis and confocal microscopic imaging. PECAM-1+ and vWF+ vascular-like structures were observed in both in vitro and in vivo angiogenesis models. High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC. The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues. Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases.en_US
dc.identifier.doi10.1111/jcmm.12051
dc.identifier.epage692
dc.identifier.issue5
dc.identifier.scopusWOS:000319515300012
dc.identifier.spage681
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12165
dc.identifier.volume17
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Cellular And Molecular Medicineen_US
dc.sourceWeb Of Science (ISI)
dc.subjectfetal stem cellsen_US
dc.subjectpro-angiogenicen_US
dc.subjectquantitative PCRen_US
dc.subjectvascularen_US
dc.subjectischemic diseaseen_US
dc.titlePro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluationen_US
dc.typeArticleen_US
dspace.entity.typePublication

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