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Antifungal activity of lactic acid bacteria isolated from fermented catfish (Clarias gariepinus) as biocontrol of sclerotium rolfsii infecting chili plants

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dc.FundingDetailsMinistry of Higher Education, Malaysia,�MOHE
dc.FundingDetailsAuthors thank to the Ministry of Higher Education, Malaysia for providing financial support to carry out this project through Exploratory Research Grant Scheme (ERGS), Vot. No 55087.
dc.contributor.affiliationsFaculty of Science and Technology
dc.contributor.affiliationsUniversiti Malaysia Terengganu (UMT)
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorZakaria S.F.en_US
dc.contributor.authorLani M.N.en_US
dc.contributor.authorSeng C.T.en_US
dc.contributor.authorAhmad F.en_US
dc.contributor.authorAhmad K.M.en_US
dc.contributor.authorHassan Z.en_US
dc.date.accessioned2024-05-28T08:24:27Z
dc.date.available2024-05-28T08:24:27Z
dc.date.issued2018
dc.description.abstractSclerotium rolfsii is one of the most destructive soil-borne pathogen of chili plant (Capsicum annum) causing damping-off, stem rot, root rot, southern blight and wilt diseases. Severely infected chili plant diseases could reduce the quality and quantity of chili produce resulting in economic loss. Lactic Acid Bacteria (LAB) are reported to have antifungal activity. However, very little information is known on antifungal activity against S. rolfsii. Hence, the objectives of this study were to identify the LAB species isolated from fermented catfish and to determine antifungal activities of the isolated LAB against S. rolfsii. Identification of LAB from fermented catfish was done by API 50 CHL and 16S rRNA gene sequencing. The identified isolates were Lactobacillus plantarum with different strains name such as JCM 1149, WCFS1 strain WCFS1 and strain NRRL B-14678. In this study, nine cultures of Cell Free Supernatant (CFS-LAB) were tested for antifungal activity on Potato Dextrose Agar (PDA). Results showed that four of Lb. plantarum strains from fermented catfish showed greater inhibitory activity (60% to 64.7%) against S. rolfsii after incubation at 28�C for three days compared with the control treatment (S. rolfsii growth in PDA without CFS-LAB). Active compound present in the crude supernatant was partially purified using ammonium sulphate precipitation method were tested at different temperatures, pH and enzymes. The proteins of LAB bacteriocins were analysed by electrophoresis and band of interest were identified by Mass Spectrometry (LC-MS/MS) based on peptide sequencing. Findings of study confirmed that the antifungal activity of Lb. plantarum was chitin-binding protein and was effectively acted as biocontrol agent against fungal infection in chili plant. � 2018, Malaysian Society of Applied Biology. All rights reserved.
dc.description.natureFinalen_US
dc.identifier.epage126
dc.identifier.issn1268643
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85056391064
dc.identifier.spage117
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85056391064&partnerID=40&md5=aaf7aa76baed768530a989c5b8a739f2
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8484
dc.identifier.volume47
dc.languageEnglish
dc.language.isoen_US
dc.publisherMalaysian Society of Applied Biologyen_US
dc.relation.ispartofMalaysian Applied Biology
dc.sourceScopus
dc.subjectBacteriocinen_US
dc.subjectCell free supernatant (CFS)en_US
dc.subjectChitin-binding proteinen_US
dc.subjectFishen_US
dc.subjectLben_US
dc.subjectPlantarumen_US
dc.titleAntifungal activity of lactic acid bacteria isolated from fermented catfish (Clarias gariepinus) as biocontrol of sclerotium rolfsii infecting chili plants
dc.typeArticleen_US
dspace.entity.typePublication

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