Antibacterial properties of clinacanthus nutans extracts against porphyromonas gingivalis and aggregatibacter actinomycetemcomitans: An in-vitro study

The aim of this study is to futher investigate and validate the antibacterial effect of Clinacanthus nutans plant extract against periodontal pathogens namely Porphyromonas gingivalis and Aggregatibacter actinomycetamcomitans. Four samples of alcoholic extract of C. nutans leaves were used in different concentrations i.e. 100%, 50%, 10% ethanol and 100% chlorofom and Chlorhexidine 0.2% (CHX) was used as the positive control. The antibacterial activity of C.nutans extract were investigated using disc diffusion agar test for determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). In this study, 50% ethanol extract and 100% chlorofom extract were found to have antibacterial activity against P.gingivalis, while only 50% ethanol crude extract was found to have acceptable antibacterial activity against A. actinomycetamcomitans (p < 0.05). The MIC and MBC tests showed that 50% ethanol extract had bacteriostatic activity against both P.gingivalis and A. actinomycetamcomitans while 100% chlorofom extract had bactericidal activity against P. gingivalis. These two findings were also found to be better than the activity of CHX. C. nutans extract was found to have notable antibacterial activity against P. gingivalis and A. actinomycetamcomitans comparable to CHX 0.2%. � 2019 Journal of International Dental and Medical Research.