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Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development

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dc.contributor.authorOng, EBBen_US
dc.contributor.authorAnthony, AAen_US
dc.contributor.authorIsmail, Aen_US
dc.contributor.authorIsmail, Aen_US
dc.contributor.authorLim, TSen_US
dc.date.accessioned2024-05-29T03:26:24Z
dc.date.available2024-05-29T03:26:24Z
dc.date.issued2013
dc.description.abstractThe hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. (c) 2013 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.diagmicrobio.2013.05.010
dc.identifier.epage89
dc.identifier.issn0732-8893
dc.identifier.issue1
dc.identifier.scopusWOS:000323467700018
dc.identifier.spage87
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12108
dc.identifier.volume77
dc.languageEnglish
dc.language.isoen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofDiagnostic Microbiology And Infectious Disease
dc.sourceWeb Of Science (ISI)
dc.subjectSalmonellaen_US
dc.subjectTyphien_US
dc.subjectHlyEen_US
dc.subjectAntigenen_US
dc.subjectImmunoblot, ELISAen_US
dc.titleCloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
dc.typeArticleen_US
dspace.entity.typePublication

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