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Multiplex STR panel for assessment of chimerism following hematopoietic stem cell transplantation (HSCT)

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dc.contributor.authorChia, WCen_US
dc.contributor.authorKhoo, TSen_US
dc.contributor.authorWahid, SFSAen_US
dc.contributor.authorRazak, NFAen_US
dc.contributor.authorAlauddin, Hen_US
dc.contributor.authorSabudin, RZARen_US
dc.contributor.authorOthman, Aen_US
dc.contributor.authorHassan, Ren_US
dc.contributor.authorHussin, NHen_US
dc.date.accessioned2024-05-29T02:59:47Z
dc.date.available2024-05-29T02:59:47Z
dc.date.issued2019
dc.descriptionAnnals of Hematology volume 98, pages1279–1291(2019)en_US
dc.description.abstractShort tandem repeat (STR) analysis is used in chimerism monitoring after allogeneic hematopoietic stem cell transplantation (HSCT) for patients with various hematologic malignancies. Commercial forensic STR kits often contain loci with huge differences in power of discrimination (PD) across populations, causing some loci to be less informative for chimerism analysis in certain populations. This study aimed to construct a new STR multiplex panel with highly informative loci for efficient chimerism analysis. Thirteen STR markers which exhibit high PD (> 0.9) in at least 80% of 50 populations globally were selected to form a new panel and used in STR analysis of 253 Malaysian subjects. Cumulative power of discrimination (CPD) and combined power of exclusion (CPE) were determined from 253 Malaysian individuals. Loci informativity was assessed and compared to the commercial AmpFLSTR Identifiler PCR Amplification kit in 14 donor-recipient pairs. The new panel had detected 202 unique alleles including five novel alleles from the 253 individuals with high CPD and CPE (> 0.99999999999999999 and > 0.999999997 respectively). All loci from the new panel in the donor-recipient pair analysis showed higher than 50% informativity, while five loci from the commercial kit demonstrated lower than 50% informativity. Four loci from the new panel ranked the highest informativity. A sequenced allelic ladder which consists of 202 unique alleles from the 253 subjects was also developed to ensure accurate allele designation. The new 13-loci STR panel, thus, could serve as an additional powerful, accurate, and highly informative panel for chimerism analysis for HSCT patients.en_US
dc.identifier.doi10.1007/s00277-019-03626-w
dc.identifier.epage1291
dc.identifier.isbn1432-0584
dc.identifier.issn0939-5555
dc.identifier.issue5
dc.identifier.scopusWOS:000464721200023
dc.identifier.spage1279
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85061708739&doi=10.1007%2fs00277-019-03626-w&partnerID=40&md5=a7f280cd4959e8390930774037b64afe
dc.identifier.urihttps://link.springer.com/article/10.1007/s00277-019-03626-w
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11753
dc.identifier.volume98
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherSpringeren_US
dc.relation.ispartofAnnals Of Hematologyen_US
dc.sourceWeb Of Science (ISI)
dc.subjectShort tandem repeats (STR)en_US
dc.subjectMicrosatelliteen_US
dc.subjectHematopoietic stem cell transplantation (HSCT)en_US
dc.subjectChimerismen_US
dc.subjectPolymerase chain reaction (PCR)en_US
dc.subjectMultiplex PCRen_US
dc.titleMultiplex STR panel for assessment of chimerism following hematopoietic stem cell transplantation (HSCT)en_US
dc.typeArticleen_US
dspace.entity.typePublication

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