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Fluorescence bioanalytical method for urea determination based on water soluble ZnS quantum dots

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dc.FundingDetailsUniversiti Kebangsaan Malaysia: DPP-2016-064,�DIP-2014-016
dc.FundingDetailsWe acknowledge financial support from Universiti Kebangsaan Malaysia via grants DPP-2016-064 and DIP-2014-016. Eka Safitri is now currently pursuing her Ph.D. degree at the School of Chemical Sciences and Food Technology, of Universiti Kebangsaan Malaysia. She is now a lecturer at the Chemistry Department, Faculty Mathematics and Natural Sciences, Syiah Kuala University (USK). Her research interests include electrochemistry, nanotechnology, sensor and biosensor. Lee Yook Heng received his B.Sc in chemistry from the Universiti Kebangsaan Malaysia. He also obtained both M.Sc. and Ph.D. from the University of Cambridge, United Kingdom. He is now a professor of bioanalytical chemistry at the School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia and carrying out research in the fields of electrochemistry, nanotechnology, sensor and biosensor.
dc.citedby7
dc.contributor.affiliationsFaculty of Science and Technology
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsSyiah Kuala University (USK)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorSafitri E.en_US
dc.contributor.authorHeng L.Y.en_US
dc.contributor.authorAhmad M.en_US
dc.contributor.authorLing T.L.en_US
dc.date.accessioned2024-05-28T08:35:30Z
dc.date.available2024-05-28T08:35:30Z
dc.date.issued2017
dc.description.abstractA new bioanalytical method for urea determination by using the immobilized urease on the highly luminescent ZnS quantum dots (QDs), which functioned as the pH fluorescent label has been developed. Bioconjugation of ZnS QDs to urease via amide bond was performed to obtain the ZnS QDs-urease bioconjugate. Upon enzymatic hydrolysis of urea by the immobilized urease, a pH change to a more alkaline condition has led to the deprotonation of ZnS QDs, and an increase in the fluorescence intensity can be observed. The fluorescence intensity of the urease conjugated ZnS QDs changed linearly with the urea concentrations between 4 � 10?9 M and 4 � 10?3 M (R2 = 0.992) at pH 6 with a calibration sensitivity of 179.46 intensity/decade. No noticeable influence by the Ca2+, Mg2+, K+ and Na+ ions on the response of the fluorescent pH bioprobe. Urea determination in soil sample by using the urea bioprobe was in good agreement with the standard DMAB (p-dimethylaminobenzaldehyde) UV�vis spectrophotometric method. By employing the strategy of ZnS QDs-urease bioconjugation, the stable covalent link between the two substances has appeared to widen the dynamic range and lower the detection limit for urea compared to free enzyme and QDs in solution for bioassay of urea concentration. � 2016
dc.description.natureFinalen_US
dc.identifier.CODENSABCE
dc.identifier.doi10.1016/j.snb.2016.08.129
dc.identifier.epage769
dc.identifier.issn9254005
dc.identifier.scopus2-s2.0-84990948089
dc.identifier.spage763
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84990948089&doi=10.1016%2fj.snb.2016.08.129&partnerID=40&md5=2242d72c31037c2ed7551832a250c7f5
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9127
dc.identifier.volume240
dc.languageEnglish
dc.language.isoen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofSensors and Actuators, B: Chemical
dc.sourceScopus
dc.subjectFluorescent labelen_US
dc.subjectQuantum dotsen_US
dc.subjectUrea biosensoren_US
dc.subjectUrease immobilizationen_US
dc.subjectZnSen_US
dc.subjectAlkalinityen_US
dc.subjectBioassayen_US
dc.subjectEnzymatic hydrolysisen_US
dc.subjectFluorescenceen_US
dc.subjectMetabolismen_US
dc.subjectNanocrystalsen_US
dc.subjectSpectrophotometryen_US
dc.subjectUreaen_US
dc.subjectZincen_US
dc.subjectZinc sulfideen_US
dc.subjectAlkaline conditionsen_US
dc.subjectBioanalytical methodsen_US
dc.subjectFluorescence intensitiesen_US
dc.subjectFluorescent labelsen_US
dc.subjectImmobilized ureaseen_US
dc.titleFluorescence bioanalytical method for urea determination based on water soluble ZnS quantum dots
dc.typeArticleen_US
dspace.entity.typePublication

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