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Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides

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dc.contributor.authorAzman A.R.en_US
dc.contributor.authorMahat N.A.en_US
dc.contributor.authorAbdul Wahab R.en_US
dc.contributor.authorAhmad W.A.en_US
dc.contributor.authorMohamed Huri M.A.en_US
dc.contributor.authorAbdul Hamid A.A.en_US
dc.contributor.authorAdamu A.en_US
dc.contributor.authorMat Saat G.A.en_US
dc.date.accessioned2024-05-29T02:00:35Z
dc.date.available2024-05-29T02:00:35Z
dc.date.issued2020
dc.description.abstractConsidering the significant evidential values of fingerprints in underwater criminal investigations and the need to visualise them using a user- and environmentally-friendly reagent, development of a novel, rapid and relatively greener nanobio-based reagent (NBR) is deemed beneficial. Lipase from the commercial Candida rugosa immobilised onto acid-functionalised multi-walled carbon nanotubes (NBR) was used as the safer and cheap lipid-sensing reagent to visualise groomed whole/split fingerprints on non-porous objects immersed in stagnant tap water for up to 30 days under a laboratory-controlled setting. Attenuated Total Reflectance – Fourier Transform Spectrometry, Field Emission Scanning Electron Microscopy and bioinformatics (molecular docking and molecular dynamics simulations) were employed to characterise and confirm the attachment of NBR onto the lipid constituents of wet fingerprints. Chromatographic results further confirmed the presence of n-hexadecanoic and octadecanoic acids on fingerprints up to 30 days of immersion. Thus, NBR may potentially be useful as the future state-of-the-art fingerprint visualisation technology. © 2020 Elsevier Ltden_US
dc.identifier.doi10.1016/j.procbio.2020.05.033
dc.identifier.epage112
dc.identifier.issn13595113
dc.identifier.scopus2-s2.0-85086410409
dc.identifier.spage102
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85086410409&doi=10.1016%2fj.procbio.2020.05.033&partnerID=40&md5=757c0899a82b6f8b7670a7fa6443ec6b
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10128
dc.identifier.volume96
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofAnatomy and Cell Biologyen_US
dc.sourceScopus
dc.subjectBioinformaticsen_US
dc.subjectCandida rugosa lipaseen_US
dc.subjectForensic scienceen_US
dc.subjectLatent fingerprinten_US
dc.subjectNanobio-based reagenten_US
dc.titleCharacterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slidesen_US
dc.typeArticleen_US
dspace.entity.typePublication

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