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Antibacterial And Antibiofilm Analyses Of Melastoma Malabathricum Leaves Extract Against Streptococcus Mutans On Tooth Surfaces

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dc.contributor.authorFatin Farhana Azizanen_US
dc.contributor.authorRohazila Mohamad Hanafiahen_US
dc.contributor.authorMarwan Jawad Msarahen_US
dc.contributor.authorNorefrina Shafinaz Md. Noren_US
dc.contributor.authorNazlina Ibrahimen_US
dc.contributor.authorWan Syaidatul Aqmaen_US
dc.date.accessioned2024-05-28T05:52:37Z
dc.date.available2024-05-28T05:52:37Z
dc.date.issued2020
dc.date.submitted15/12/2020
dc.descriptionVolume :16 No:6en_US
dc.description.abstractAims: Dental caries is a chronic infectious disease caused by Streptococcus mutans due to its ability to form biofilm. This study aims to assess the antimicrobial efficacy of Melastoma malabathricum leaf extract against S. mutans on the surface of tooth samples as a potential therapy for dental caries. Methodology and results: Extraction of M. malabathricum leaves was done using acetone as the solvent and antibacterial activity of the extracts was determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Antibiofilm activity of M. malabathricum extract against S. mutans was determined by comparing the colony count, biofilm formation assay and morphology observation by scanning electron microscope (SEM). The MIC value of extracts was 6.25 mg/mL and MBC value was >25 mg/mL. A decrease in colony count was noted when tooth samples were incubated with M. malabathricum extract for 8 h compared to 4 h incubation. At pH 5, the formation of the colony was the least, medium at pH 8 and maximum at pH 7. A decrease in biofilm formation was observed when tooth samples were incubated with the extract for 8 h. SEM observations showed treatment with the extract caused S. mutans cell membrane to leak leading to cell morphology changes. Conclusion, significance and impact of study: Acetone extract of M. malabathricum leaves showed excellent antibacterial activity against S. mutans. It has bactericidal activity with the ability to inhibit biofilm in dose-dependent manner against S. mutans. The morphological analyses suggested that the extract disrupted the cell membrane of the bacteria.en_US
dc.identifier.doihttp://dx.doi.org/10.21161/mjm.190678
dc.identifier.epage460
dc.identifier.issn2231-7538
dc.identifier.issue6
dc.identifier.spage454
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/6725
dc.identifier.volume16
dc.language.isoen_USen_US
dc.publisherMalaysian Society for Microbiologyen_US
dc.relation.ispartofMalaysian Journal of Microbiologyen_US
dc.subjectantibacterial, biofilm, Melastoma malabathricum, Streptococcus mutansen_US
dc.titleAntibacterial And Antibiofilm Analyses Of Melastoma Malabathricum Leaves Extract Against Streptococcus Mutans On Tooth Surfacesen_US
dc.typeArticleen_US
dspace.entity.typePublication

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