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Organotypic culture of human amnion cells in air-liquid interface as a potential substitute for skin regeneration

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dc.Chemicals/CASfibrin, 9001-31-4
dc.FundingDetails02�01�02�SF0289 Universiti Kebangsaan Malaysia Ministry of Science, Technology and Space
dc.FundingDetailsThis study was funded by the Ministry of Science,�Technology and Innovations , Grant No. 02�01�02�SF0289 . Fatimah Simat Siti is our PhD student in Universiti Kebangsaan Malaysia and is a National Science Fellowship scholar of Ministry of Science, Technology and Innovations. We thank Megat Radzi Megat A. Rani, Rafiuz Zaman Haroun and Mohamad Hasnul Naim Abd Hamid for technical expertise in electron microscopy. We also thank the Electron Microscopy Unit, Faculty of Science and Technology, UKM and Microscopy Unit, Institute of Bioscience, UPM. Disclosure of interests: The authors have no commercial, proprietary, or financial interest in the products or companies described in this article.
dc.citedby6
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Malaysia Sabah (UMS)
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorFatimah S.S.en_US
dc.contributor.authorChua K.en_US
dc.contributor.authorTan G.C.en_US
dc.contributor.authorAzmi T.I.en_US
dc.contributor.authorTan A.E.en_US
dc.contributor.authorRahman H.A.en_US
dc.date.accessioned2024-05-28T08:33:30Z
dc.date.available2024-05-28T08:33:30Z
dc.date.issued2013
dc.description.abstractBackground aims: The aim of the present study was to evaluate the effects of air-liquid interface on the differentiation potential of human amnion epithelial cells (HAECs) to skin-like substitute in organotypic culture. Methods: HAECs at passage 1e2 were seeded onto a fibrin layer populated with human amnion mesenchymal cells to form the organotypic cultures. The organotypic HAECs were then cultured for 7, 14 and 21 d in two types of culture system: the submerged culture and the airliquid interface culture. Cell morphogenesis was examined under the light and electron microscopes (transmission and scanning) and analyzed by immunohistochemistry. Results: Organotypic HAECs formed a single layer epithelium after 3 wk in submerged as well as air-liquid interface cultures. Ultrastructurally, desmosomes were observed in organotypic HAECs cultured in the air-liquid interface but not in the submerged culture. The presence of desmosomes marked the onset of early epidermal differentiation. Organotypic HAECs were positive against anti-CK18 and anti-CK14 in both the submerged and the air-liquid interface cultures. The co-expression of CK14 and CK18 suggested that differentiation of HAECs into skin may follow the process of embryonic skin development. However, weak expression of CK14 was observed after 2 and 3 wk of culture in air-liquid interface. CK10, involucrin, type IV collagen and laminin-5 expression was absent in organotypic HAECs. This observation reflects the initial process of embryonic epidermal differentiation and stratification. Conclusions: Results from the present study suggest that the air-liquid interface could stimulate early differentiation of organotypic HAECs to epidermal cells, with a potential use for skin regeneration. � 2013, International Society for Cellular Therapy.en_US
dc.description.natureFinalen_US
dc.identifier.CODENCYTRF
dc.identifier.doi10.1016/j.jcyt.2013.05.003
dc.identifier.epage1041
dc.identifier.issn14653249
dc.identifier.issue8
dc.identifier.pmid23830235
dc.identifier.scopus2-s2.0-84890901867
dc.identifier.spage1030
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84890901867&doi=10.1016%2fj.jcyt.2013.05.003&partnerID=40&md5=a4b43a7b4089786017ab4906fc89678a
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9060
dc.identifier.volume15
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherElsevier Inc.en_US
dc.relation.ispartofCytotherapyen_US
dc.sourceScopus
dc.subjectAir-liquid interfaceen_US
dc.subjectEpithelial stem cellsen_US
dc.subjectFibrinen_US
dc.subjectHuman amnionederived stem cellsen_US
dc.subjectOrganotypic cultureen_US
dc.subjectSkin regenerationen_US
dc.titleOrganotypic culture of human amnion cells in air-liquid interface as a potential substitute for skin regenerationen_US
dc.title.alternativeCytotherapyen_US
dc.typeArticleen_US
dspace.entity.typePublication

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