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Antioxidative effects of germinated brown rice-derived extracts on H2O 2-induced oxidative stress in HepG2 cells

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dc.Chemicals/CAS4 aminobutyric acid, 28805-76-7, 56-12-2; gamma oryzanol, 11042-64-1; hydrogen peroxide, 7722-84-1; hydroxyl radical, 3352-57-6; protein kinase B, 148640-14-6; stress activated protein kinase, 155215-87-5; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1
dc.FundingDetailsUniversiti Putra Malaysia,�UPM
dc.citedby6
dc.contributor.affiliationsFaculty of Dentistry
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorNur Diyana Md Zamrien_US
dc.contributor.authorMustapha Umar Imamen_US
dc.contributor.authorSiti Aisyah Abd Ghafaren_US
dc.contributor.authorMaznah Ismailen_US
dc.date.accessioned2024-05-28T08:32:14Z
dc.date.available2024-05-28T08:32:14Z
dc.date.issued2014
dc.description.abstractThe antioxidant properties of germinated brown rice (GBR) are likely mediated by multiple bioactives. To test this hypothesis, HepG2 cells pretreated with GBR extracts, rich in acylated steryl glycoside (ASG), gamma amino butyric acid GABA), phenolics or oryzanol, were incubated with hydrogen peroxide (H2O2) and their hydroxyl radical (OH•) scavenging capacities and thiobarbituric acid-reactive substances (TBARS) generation were evaluated. Results showed that GBR-extracts increased OH• scavenging activities in both cell-free medium and posttreatment culture media, suggesting that the extracts were both direct- And indirect-acting against OH•. The levels of TBARS in the culture medium after treatment were also reduced by all the extracts. In addition, H2O2 produced transcriptional changes in p53, JNK, p38 MAPK, AKT, BAX, and CDK4 that were inclined towards apoptosis, while GBR-extracts showed some transcriptional changes (upregulation of BAX and p53) that suggested an inclination for apoptosis although other changes (upregulation of antioxidant genes, AKT, JNK, and p38 MAPK) suggested that GBR-extracts favored survival of the HepG2 cells. Our findings show that GBR bioactive-rich extracts reduce oxidative stress through improvement in antioxidant capacity, partly mediated through transcriptional regulation of antioxidant and prosurvival genes.en_US
dc.description.natureFinalen_US
dc.identifier.ArtNo371907
dc.identifier.doi10.1155/2014/371907
dc.identifier.issn1741427X
dc.identifier.scopus2-s2.0-84934934675
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84934934675&doi=10.1155%2f2014%2f371907&partnerID=40&md5=b8ad5aecadb639ed2ec7a747bb1280f0
dc.identifier.urihttps://www.hindawi.com/journals/ecam/2014/371907/
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9015
dc.identifier.volume2014
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherHindawi Limiteden_US
dc.relation.ispartofOpen Accessen_US
dc.relation.ispartofEvidence-based Complementary and Alternative Medicine
dc.sourceScopus
dc.titleAntioxidative effects of germinated brown rice-derived extracts on H2O 2-induced oxidative stress in HepG2 cellsen_US
dc.title.alternativeEvid.-Based Complement. Altern. Med.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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