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Human mesenchymal stromal cells could deliver erythropoietin and migrate to the basal layer of hair shaft when subcutaneously implanted in a murine model

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dc.Chemicals/CASerythropoietin, 11096-26-7; hemoglobin, 9008-02-0
dc.FundingDetailsMalaysia Toray Science Foundation Majlis Kanser Nasional
dc.FundingDetailsWe acknowledge the generous support from Malaysia Toray Science Foundation (MTSF) , Scientex Foundation and National Cancer Council (MAKNA) of Malaysia. We thank the staff and fellow researchers from the Cell Banking Unit, Genetic Cancer Laboratory, Hemostasis Laboratory, Tissue Engineering Laboratory, Hematology Laboratory and Histopathology Laboratory of Universiti Kebangsaan Malaysia Medical Center, and Animal Holding Facility in Universiti Kebangsaan Malaysia Bangi for their technical guidance and assistance. We are grateful to Dr. Naseem from Histopathology Department of UKM Medical Center for reading the slides, and Professor Choo Kong Bung of Universiti Tunku Abdul Rahman for editorial comments on the manuscript.
dc.citedby8
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Tunku Abdul Rahman (UTAR)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorMok P.L.en_US
dc.contributor.authorCheong S.K.en_US
dc.contributor.authorLeong C.F.en_US
dc.contributor.authorChua K.H.en_US
dc.contributor.authorAinoon O.en_US
dc.date.accessioned2024-05-29T02:01:17Z
dc.date.available2024-05-29T02:01:17Z
dc.date.issued2012
dc.description.abstractMesenchymal stromal cells (MSC) are an attractive cell-targeting vehicle for gene delivery. MIDGE (an acronym for Minimalistic, Immunologically Defined Gene Expression) construct is relatively safer than the viral or plasmid expression system as the detrimental eukaryotic and prokaryotic gene and sequences have been eliminated. The objective of this study was to test the ability of the human MSC (hMSC) to deliver the erythropoietin (EPO) gene in a nude mice model following nucleofection using a MIDGE construct. hMSC nucleofected with MIDGE encoding the EPO gene was injected subcutaneously in Matrigel at the dorsal flank of nude mice. Subcutaneous implantation of nucleofected hMSC resulted in increased hemoglobin level with presence of human EPO in the peripheral blood of the injected nude mice in the first two weeks post-implantation compared with the control groups. The basal layer of the hair shaft in the dermal layer was found to be significantly positive for immunohistochemical staining of a human EPO antibody. However, only a few basal layers of the hair shaft were found to be positively stained for CD105. In conclusion, hMSC harboring MIDGE-EPO could deliver and transiently express the EPO gene in the nude mice model. These cells could be localized to the hair follicle and secreted EPO protein might have possible role in hair regeneration. � 2012 Elsevier Ltd.en_US
dc.description.natureFinalen_US
dc.identifier.CODENTICEB
dc.identifier.doi10.1016/j.tice.2012.04.002
dc.identifier.epage256
dc.identifier.issn408166
dc.identifier.issue4
dc.identifier.pmid22560724
dc.identifier.scopus2-s2.0-84861996223
dc.identifier.spage249
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84861996223&doi=10.1016%2fj.tice.2012.04.002&partnerID=40&md5=22b9f084c6c7e6840bab6950272d9df4
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10160
dc.identifier.volume44
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofTissue and Cellen_US
dc.sourceScopus
dc.subjectErythropoietinen_US
dc.subjectHair regenerationen_US
dc.subjectHemoglobinen_US
dc.subjectMesenchymal stromal cellsen_US
dc.subjectMIDGEen_US
dc.subjectNucleofectionen_US
dc.titleHuman mesenchymal stromal cells could deliver erythropoietin and migrate to the basal layer of hair shaft when subcutaneously implanted in a murine modelen_US
dc.title.alternativeTissue Cellen_US
dc.typeArticleen_US
dspace.entity.typePublication

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