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Effects of environmental and growth conditions on protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil

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dc.FundingDetailsUniversiti Sains Islam Malaysia Ministry of Higher Education, Malaysia,�MOHE: FRGS/1/2015/SG05/USIM/02/1,�USIM/FRGS/ FST/32/51915
dc.FundingDetailsThe authors would like to thank the Ministry of Higher Education of Malaysia for the financial support through FRGS Grant Scheme USIM/FRGS/ FST/32/51915 or FRGS/1/2015/SG05/USIM/02/1 and facilities through Universiti Sains Islam Malaysia.
dc.contributor.affiliationsFaculty of Science and Technology
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorNoor H.M.en_US
dc.contributor.authorAlhadi N.A.en_US
dc.contributor.authorMohamed H.S.en_US
dc.contributor.authorRehan M.M.en_US
dc.contributor.authorRadzi S.M.en_US
dc.date.accessioned2024-05-28T08:24:33Z
dc.date.available2024-05-28T08:24:33Z
dc.date.issued2018
dc.description.abstractThe presence of antimicrobial substances at low concentration may act as chemical signals in altering metabolic processes that take place in bacteria. The chemical signals may activate lots of signal transduction that result in the secretion of bioactive proteins by the stimulated bacterial cells. The bioactive proteins production is strongly influenced by certain factors such as pH and nutrient sources. Therefore, the study of environmental stress factors and growth conditions is very important in inducing the production of bioactive proteins by microbes. The main objective of this study is to evaluate the effects of environmental stress factors and growth conditions on the production of bioactive proteins by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil. The microbial proteins were produced by fermentation process using Nutrient broth with adjustment of pH media (pH 6, 7 and 8), and supplementation with 1% (w/w) of three different nutrients, including carbon sources (glucose, sucrose and starch), nitrogen sources (urea, casein and gelatin) and inorganic salts (calcium chloride, sodium nitrate, sodium dihydrogen phosphate) separately. The extracellular protein produced by B. subtilis ATCC21332 was isolated and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to detect appearance of new protein bands. Results showed that new extracellular proteins were synthesized by B. subtilis ATCC21332 after being induced with C. flexuosus essential oil and grown in each media with 1% (w/w) of starch, 1% (w/w) of gelatin, 1% (w/w) of casein, 1% (w/w) of sodium dihydrogen phosphate and 1% (w/w) of calcium chloride with approximate size of 30.43 kDa, 30.66 kDa, 31.92 kDa, 31.35 kDa and 30.80 kDa respectively. Hence, B. subtilis ATCC21332 in the presence of 0.01 MIC C. flexuosus essential oil under different conditions are able to produce or secrete new extracellular proteins. � 2018, Malaysian Society of Applied Biology. All rights reserved.en_US
dc.description.natureFinalen_US
dc.identifier.citationMOHD NOOR, H., AHMAD ALHADI, N., MOHAMED, H. S. ., REHAN, M. M., & MAT RADZI, S. (2018). EFFECTS OF ENVIRONMENTAL AND GROWTH CONDITIONS ON PROTEIN PRODUCED BY Bacillus subtilis ATCC21332 IN THE PRESENCE OF Cymbopogon flexuosus ESSENTIAL OIL. Malaysian Applied Biology, 47(2), 103–111. Retrieved from https://jms.mabjournal.com/index.php/mab/article/view/1953en_US
dc.identifier.epage111
dc.identifier.issn1268643
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85047666134
dc.identifier.spage103
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85047666134&partnerID=40&md5=39fafdec94fdec3073de405b9377b7b8
dc.identifier.urihttps://jms.mabjournal.com/index.php/mab/article/view/1953
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8516
dc.identifier.volume47
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherMalaysian Society of Applied Biologyen_US
dc.relation.ispartofMalaysian Applied Biologyen_US
dc.sourceScopus
dc.subjectBacillus subtilis ATCC21332en_US
dc.subjectCymbopogon flexuosusen_US
dc.subjectEnvironmental and growth conditionen_US
dc.subjectEssential oilen_US
dc.subjectProteinen_US
dc.titleEffects of environmental and growth conditions on protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oilen_US
dc.typeArticleen_US
dspace.entity.typePublication

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