Analysis of Antimicrobial Compound in Ziziphus Mauritiana Extract using Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR)

The usage of herbal medicinal is widely used among society today in daily lives. Ziziphus mauritiana locally known as Bidara belonging to family Rhamnaceae is a herbal plant that believed originated from Indo-Malaysian region of South-East Asia. It is commonly known for its various added values in term of pharmacological such as antioxidants and antimicrobial. To prove its efficacy, antimicrobial compound analysis of Ziziphus mauritiana leaf extract was performed using Attenuated total reflection-fourier transform infrared (ATR-FTIR). The spectrum and wavelength obtained can be used to identify the functional group and thus, the antimicrobial compound present in the plant. A liquid-liquid extraction had been carried out to obtain the extract of Ziziphus mauritiana leaf sample and rotary evaporator is used to remove the solvent of ethyl acetate. Based on this study, Ziziphus mauritiana leaves do have antimicrobial compounds such as eugenol and vanillin which are one of the antimicrobial agents.