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Occurrence of Blastocystis sp. in water catchments at Malay villages and Aboriginal settlement during wet and dry seasons in Peninsular Malaysia

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dc.Chemicals/CASchlorine, 13981-72-1, 7782-50-5; sulfate, 14808-79-8
dc.citedby7
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM) Medical Centre
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsKolej Universiti Insaniah
dc.contributor.affiliationsUniversiti Teknologi MARA (UiTM)
dc.contributor.authorNoradilah S.A.en_US
dc.contributor.authorLee I.L.en_US
dc.contributor.authorAnuar T.S.en_US
dc.contributor.authorSalleh F.M.en_US
dc.contributor.authorManap S.N.A.A.en_US
dc.contributor.authorMohtar N.S.H.M.en_US
dc.contributor.authorAzrul S.M.en_US
dc.contributor.authorAbdullah W.O.en_US
dc.contributor.authorMoktar N.en_US
dc.date.accessioned2024-05-28T08:33:54Z
dc.date.available2024-05-28T08:33:54Z
dc.date.issued2016
dc.description.abstractIn the tropics, there are too few studies on isolation of Blastocystis sp. subtypes from water sources; in addition, there is also an absence of reported studies on the occurrence of Blastocystis sp. subtypes in water during different seasons. Therefore, this study was aimed to determine the occurrence of Blastocystis sp. subtypes in river water and other water sources that drained aboriginal vicinity of highly endemic intestinal parasitic infections during wet and dry seasons. Water samples were collected from six sampling points of Sungai Krau (K1-K6) and a point at Sungai Lompat (K7) and other water sources around the aboriginal villages. The water samples were collected during both seasons, wet and dry seasons. Filtration of the water samples were carried out using a flatbed membrane filtration system. The extracted DNA from concentrated water sediment was subjected to single round polymerase chain reaction and positive PCR products were subjected to sequencing. All samples were also subjected to filtration and cultured on membrane lactose glucuronide agar for the detection of faecal coliforms. During wet season, Blastocystis sp. ST1, ST2 and ST3 were detected in river water samples. Blastocystis sp. ST3 occurrence was sustained in the river water samples during dry season. However Blastocystis sp. ST1 and ST2 were absent during dry season. Water samples collected from various water sources showed contaminations of Blastocystis sp. ST1, ST2, ST3 and ST4, during wet season and Blastocystis sp. ST1, ST3, ST8 and ST10 during dry season. Water collected from all river sampling points during both seasons showed growth of Escherichia coli and Enterobacter aerogenes, indicating faecal contamination. In this study, Blastocystis sp. ST3 is suggested as the most robust and resistant subtype able to survive in any adverse environmental condition. Restriction and control of human and animal faecal contaminations to the river and other water sources shall prevent the transmission of Blastocystis sp. to humans and animals in this aboriginal community. � 2016 Noradilah et al.en_US
dc.description.natureFinalen_US
dc.identifier.ArtNoe2541
dc.identifier.doi10.7717/peerj.2541
dc.identifier.issn21678359
dc.identifier.issue10
dc.identifier.scopus2-s2.0-84992144631
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84992144631&doi=10.7717%2fpeerj.2541&partnerID=40&md5=5661bb941228d20826c353c6a242a886
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9073
dc.identifier.volume2016
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherPeerJ Inc.en_US
dc.relation.ispartofOpen Accessen_US
dc.relation.ispartofPeerJ
dc.sourceScopus
dc.subjectAboriginal settlementsen_US
dc.subjectBlastocystis sp.en_US
dc.subjectWateren_US
dc.subjectWet and dry seasonsen_US
dc.titleOccurrence of Blastocystis sp. in water catchments at Malay villages and Aboriginal settlement during wet and dry seasons in Peninsular Malaysiaen_US
dc.title.alternativePeerJen_US
dc.typeArticleen_US
dspace.entity.typePublication

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