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Pulchrin A, a New Natural Coumarin Derivative of Enicosanthellum pulchrum, Induces Apoptosis in Ovarian Cancer Cells via Intrinsic Pathway

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dc.contributor.authorNoraziah Nordinen_US
dc.contributor.authorMehran Fadaeinasaben_US
dc.contributor.authorSyam Mohanen_US
dc.contributor.authorNajihah Mohd Hashimen_US
dc.contributor.authorRozana Othmanen_US
dc.contributor.authorHamed Karimianen_US
dc.contributor.authorVenus Imanen_US
dc.contributor.authorNoorlela Ramlien_US
dc.contributor.authorHapipah Mohd Alien_US
dc.contributor.authorNazia Abdul Majiden_US
dc.date.accessioned2024-05-28T03:51:49Z
dc.date.available2024-05-28T03:51:49Z
dc.date.issued2016
dc.description.abstractDrug resistance presents a challenge in chemotherapy and has attracted research interest worldwide and particular attention has been given to natural compounds to overcome this difficulty. Pulchrin A, a new compound isolated from natural products has demonstrated novel potential for development as a drug. The identification of pulchrin A was conducted using several spectroscopic techniques such as nuclear magnetic resonance, liquid chromatography mass spectrometer, infrared and ultraviolet spectrometry. The cytotoxicity effects on CAOV-3 cells indicates that pulchrin A is more active than cisplatin, which has an IC50 of 22.3 ?M. Significant changes in cell morphology were present, such as cell membrane blebbing and formation of apoptotic bodies. The involvement of phosphatidylserine (PS) in apoptosis was confirmed by Annexin V-FITC after a 24 h treatment. Apoptosis was activated through the intrinsic pathway by activation of procaspases 3 and 9 as well as cleaved caspases 3 and 9 and ended at the executioner pathway, with the occurrence of DNA laddering. Apoptosis was further confirmed via gene and protein expression levels, in which Bcl-2 protein was down-regulated and Bax protein was up-regulated. Furthermore, the CAOV-3 cell cycle was disrupted at the G0/G1 phase, leading to apoptosis. Molecular modeling of Bcl-2 proteins demonstrated a high- binding affinity, which inhibited the function of Bcl-2 proteins and led to cell death. Results of the current study can shed light on the development of new therapeutic agents, particularly, human ovarian cancer treatments.en_US
dc.identifier.citation: Nordin N, Fadaeinasab M, Mohan S, Mohd Hashim N, Othman R, Karimian H, et al. (2016) Pulchrin A, a New Natural Coumarin Derivative of Enicosanthellum pulchrum, Induces Apoptosis in Ovarian Cancer Cells via Intrinsic Pathway. PLoS ONE 11(5): e0154023. doi:10.1371/journal. pone.0154023en_US
dc.identifier.doi10.1371/journal.pone.0154023
dc.identifier.epage22
dc.identifier.issn1932-6203
dc.identifier.issue5
dc.identifier.other2169-5
dc.identifier.spage1
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154023
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/5168
dc.identifier.volume11
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONEen_US
dc.titlePulchrin A, a New Natural Coumarin Derivative of Enicosanthellum pulchrum, Induces Apoptosis in Ovarian Cancer Cells via Intrinsic Pathwayen_US
dc.typeArticleen_US
dspace.entity.typePublication

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