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Kinetics of surfactin production by bacillus subtilis in a 5 L Stirred-tank Bioreactor

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dc.FundingDetailsMinistry of Higher Education, Malaysia,�MOHE: PPP/USG-0216/FST/30/16716,�ERGS/1/2012/STG08/USIM/02/3 Universiti Sains Islam Malaysia,�Islamic Science University Of Malaysia
dc.FundingDetailsThe authors would like to thank Universiti Sains Islam Malaysia (USIM) and Ministry of Higher Education (MOHE), Malaysia for funding this research under the internal research grant PPP/USG-0216/FST/30/16716 and ERGS/1/2012/STG08/USIM/02/3.
dc.citedby1
dc.contributor.affiliationsFaculty of Science and Technology
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.authorMubarak M.Q.E.en_US
dc.contributor.authorJufri S.H.M.en_US
dc.contributor.authorZahar S.M.S.N.S.en_US
dc.contributor.authorKalil M.S.en_US
dc.contributor.authorHamid A.A.en_US
dc.contributor.authorMohd Hafez Mohd Isaen_US
dc.date.accessioned2024-05-28T08:30:20Z
dc.date.available2024-05-28T08:30:20Z
dc.date.issued2017
dc.description.abstractA kinetic model of bacterial growth and metabolite production can adequately explain the trends and interaction of important parameters in a fermentation process. Production of surfactin by two bacterial strains, namely, Bacillus subtilis MSH1 and Bacillus subtilis ATCC 21322, in a 5 L bioreactor was investigated using Cooper's media with 4% (v/v) glucose. The present kinetic study was carried out in order to determine the correlation between microbial cell growth, surfactin production and glucose consumption. Batch fermentation was performed by cultivation of each selected strain in a bioreactor at 30�C for 55 h. The experimental results showed production of surfactin in the culture medium after 5 and 10 h of incubation for B. subtilis ATCC 21332 and B. subtilis MSH1, respectively, at which the bacterial cells were at an early stage of the log phase. The maximum concentration of surfactin (Pmax) achieved by B. subtilis MSH1 and B. subtilis ATCC 21332 was 226.17 and 447.26 mg/L, respectively. The kinetic study of bacterial cell growth of both strains indicated that B. subtilis MSH1 had a specific growth rate (?max) of 0.224 h-1 and attained a maximum biomass concentration (Xmax) as high as 2.90 g/L after 28 h of fermentation, while B. subtilis ATCC 21332, with ?max of 0.087 h-1, attained an Xmax of 2.62 g/L after 45 h of incubation. B. subtilis MSH1 showed higher growth kinetics, thus exhibited higher values of ?max and Xmax compared with B. subtilis ATCC 21332 under identical fermentation conditions. The Pmax achieved by B. subtilis ATCC 21332 was 447.26 mg/L, two times higher than that achieved by B. subtilis MSH1 (226.17 mg/L). The results obtained provide kinetics information including values of Pmax, ?max and Xmax for better understanding of interactions of bacterial cell growth and glucose consumption towards surfactin production by a commercial strain of B. subtilis ATCC 21332 and a local isolate of B. subtilis MSH1.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.17576/jsm-2017-4601-24
dc.identifier.epage1548
dc.identifier.issn1266039
dc.identifier.issue9
dc.identifier.scopus2-s2.0-85038447597
dc.identifier.spage1541
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85038447597&doi=10.17576%2fjsm-2017-4601-24&partnerID=40&md5=852bad16c84f36dc2cfa4f1dd8cf5d51
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8943
dc.identifier.volume46
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherPenerbit Universiti Kebangsaan Malaysiaen_US
dc.relation.ispartofOpen Accessen_US
dc.relation.ispartofSains Malaysiana
dc.sourceScopus
dc.subjectBacillus subtilis ATCC 21322en_US
dc.subjectBacillus subtilis MSH1en_US
dc.subjectCooper's mediaen_US
dc.subjectKinetic studyen_US
dc.subjectSurfactin productionen_US
dc.titleKinetics of surfactin production by bacillus subtilis in a 5 L Stirred-tank Bioreactoren_US
dc.title.alternativeSains Malays.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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