Publication:
Micropropagation and Assessment of Genetic Variability of Cyclanthus bipartitus

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dc.contributor.authorNur Fauzana Mohd Kasimen_US
dc.contributor.authorHanis Nadia Yahya,en_US
dc.contributor.authorSaleh Kadziminen_US
dc.contributor.authorYahya Awangen_US
dc.date.accessioned2024-05-28T03:31:43Z
dc.date.available2024-05-28T03:31:43Z
dc.date.issued2018
dc.descriptionVolume: 17 Issue: 1en_US
dc.description.abstractBackground and Objective: Cyclanthus bipartitus is an indoor ornamental plant. It’s fertilization relies on Cyclocephala sp. (Scarabaeidae). The absence of these pollinators could lead to lack of production of seed and therefore its vegetative propagation is obligatory. This study was carried out to determine the optimum concentration of benzylaminopurine (BAP), alpha-naphthalene acetic acid (NAA) and sucrose to be used in the Murashige and Skoogs (MS) media for in vitro propagation of Cyclanthus bipartitus and to evaluate the variation in DNA sequence of regenerated plants. Materials and Methods: Explants were cultured on sterilized MS medium containing different combinations of BAP, NAA and sucrose. The preparations were cultured for 10 weeks at temperature 25-28°C under 16 h of photoperiod with light intensity of 30 μmol m–2 sec–1 and the growth of the plantlets were monitored. Somaclonal variation amongst mother plants, daughter plants and the plantlets generated from the following three subculturing at the DNA level and was analyzed by using random amplified polymorphic DNA (RAPD). Data obtained were subjected to analysis of variance (ANOVA) using PROC GLM of SAS software. Results: Multiple adventitious shoots of Cyclanthus bipartitus were induced from lamina explants cultured on MS medium supplemented with various combinations of BAP, NAA and sucrose concentrations. The highest number of shoots, tallest shoots, highest number of roots and longest root were recorded in medium with a combination of 1.0 mg L–1 BAP, 0.5 mg L–1 and 30 g L–1 sucrose. Results of DNA analysis showed that 18 out of 26 score able bands were polymorphic and eight were monomorphic, which gave 69.2% polymorphism frequency. Conclusion: Optimum concentrations of BAP, NAA and sucrose for shoot and root development in vitro propagation of Cyclanthus bipartitus was 1.0 mg L–1 BAP, 0.5 mg L–1 NAA and 30 g L–1 of sucrose. 69.2% polymorphism was detected in regenerated plants indicating genetic variation had occurred amongst somaclones.en_US
dc.identifier.doi10.3923/ajps.2018.19.26
dc.identifier.epage26
dc.identifier.issn1682-3974
dc.identifier.issue1
dc.identifier.spage19
dc.identifier.urihttps://scialert.net/abstract/?doi=ajps.2018.19.26
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/4593
dc.identifier.volume17
dc.language.isoen_USen_US
dc.publisherAsian Network for Scientific Informationen_US
dc.relation.ispartofAsian Journal of Plant Sciencesen_US
dc.subjectTissue culture, benzylaminopurine, alpha-naphthalene acetic acid, sucrose, random amplified polymorphic DNAen_US
dc.titleMicropropagation and Assessment of Genetic Variability of Cyclanthus bipartitusen_US
dc.typeArticleen_US
dspace.entity.typePublication

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